McHugh T M, Reid M E, Stites D P, Chase E S, Casavant C H
Department of Laboratory Medicine, University of California, San Francisco.
Vox Sang. 1987;53(4):231-4. doi: 10.1111/j.1423-0410.1987.tb05072.x.
We used indirect immunofluorescence and flow cytometry to detect the human erythrocyte surface antigen Gerbich. The procedure consisted of sequential erythrocyte-labeling with human antibody to Gerbich, biotinylated goat anti-human IgG and finally phycoerythrin-conjugated streptavidin. With maximal excitation of phycoerythrin at 546 nm, an increase in the fluorescence sensitivity was achieved. All erythrocytes from normal controls had detectable Gerbich antigen with little variation in antigen density between individuals. Six Gerbich-negative patients had no detectable antigen. By diluting the erythrocytes, as few as 0.1% antigen-positive cells in an antigen-negative population could be detected. These studies indicate that flow cytometry is a useful technique for the detection of erythrocyte surface antigens.
我们使用间接免疫荧光和流式细胞术来检测人类红细胞表面抗原Gerbich。该过程包括依次用抗Gerbich的人抗体、生物素化山羊抗人IgG以及最后用藻红蛋白偶联的链霉亲和素标记红细胞。在藻红蛋白的最大激发波长为546nm时，实现了荧光灵敏度的提高。所有正常对照的红细胞都能检测到Gerbich抗原，个体之间抗原密度的差异很小。6名Gerbich阴性患者未检测到抗原。通过稀释红细胞，在抗原阴性群体中低至0.1%的抗原阳性细胞也能被检测到。这些研究表明，流式细胞术是检测红细胞表面抗原的一种有用技术。
