Unité de Recherche et Développement, Institut Supérieur des Professions infirmières et Techniques de Santé, Rabat (Maroc); Laboratoire de Zoologie et Biologie Générale, Faculté des Sciences, Université Mohamed V, Rabat (Maroc).
Département de Zoologie et Écologie animale, Institut Scientifique, Université Mohamed V, Rabat (Maroc).
East Mediterr Health J. 2018 Mar 5;23(12):809-814. doi: 10.26719/2017.23.12.809.
The Anopheles maculipennis complex has been implicated in the transmission of malaria, which was endemic in Morocco until 2010. Climate change, intensification of international travel and the permanent presence of the vector increase the risk of malaria re-emergence. Using polymerase chain reaction assay (PCR), this study attempts to identify the possible presence of the Maculipennis complex in several areas of Morocco, based on the concept of risk. Eighty-six mosquito specimens were analyzed using PCR amplification of the ITS2 (Internal Transcribed Spacer 2) sequence of ribosomal DNA. The ITS2 sequences of the mosquitoes were compared to those of the 7 species of the Maculipennis complex available in GenBank. The results obtained confirm the presence of Anopheles labranchiae. In order to enable technology transfer, DNA extraction and amplification steps are presented for the first time in detail, given the fact that the technique is still unknown to several Moroccan peripheral laboratories.
冈比亚按蚊复合体与疟疾的传播有关，直到 2010 年，疟疾在摩洛哥一直流行。气候变化、国际旅行的加剧以及病媒的持续存在增加了疟疾再次出现的风险。本研究采用聚合酶链反应（PCR）检测方法，基于风险概念，尝试在摩洛哥的几个地区确定冈比亚按蚊复合体的可能存在情况。使用 PCR 扩增核糖体 DNA 的 ITS2（内部转录间隔区 2）序列对 86 只蚊子标本进行分析。将蚊子的 ITS2 序列与 GenBank 中可用的 7 种冈比亚按蚊复合体的序列进行比较。所获得的结果证实了存在斑须按蚊。为了实现技术转让，首次详细介绍了 DNA 提取和扩增步骤，因为该技术对摩洛哥的一些外围实验室来说仍然是未知的。
