Müller Tamás, Matsubara Hajime, Kubara Yuki, Horváth Ákos, Kolics Balázs, Taller János, Stéger Viktor, Kovács Balázs, Horváth László, Asturiano Juan F, Peñaranda David S, Urbányi Béla
Department of Aquaculture, Szent István University, 2100 Gödöllő, Páter K. str. 1, Hungary.
Department of Aquaculture, Szent István University, 2100 Gödöllő, Páter K. str. 1, Hungary; Laboratory of Aquaculture Science, Department of Aquatic Biology, Faculty of Bioindustry, Tokyo University of Agriculture, 196 Yasaka, Abashiri, Hokkaido 099-2493, Japan.
Theriogenology. 2018 Jun;113:153-158. doi: 10.1016/j.theriogenology.2018.02.021. Epub 2018 Mar 2.
The objective of this study was to assess impact of cryopreserved European eel sperm and Japanese eel native sperm on early fertilization, hatch, survival, and malformation rates of larvae, as well as develop molecular techniques to distinguish different eel species. Eggs from Japanese eel females (Anguilla japonica) were artificially fertilized with sperm of Japanese eel males and cryopreserved sperm from European eel (A. anguilla, extender was modified Tanaka solution and methanol as cryoprotectant). There were no statistical differences (p > 0.05) among the measured parameters such as fertilization, hatch and survival after 10 days post-hatch rates due to large individual differences. The malformation rate of larvae compared to the hatching rate was higher in cryopreserved groups than in the control indicating that the methodology needs further refinement. Genetic analyses (PCR-RFLP, PCR-HRM) proved a clear result in the detection of paternal contribution in hybridization between the Japanese and the European eel and applied PCR-HRM method is a quick and cost effective tool to identify illegally imported A. anguilla at the glass eel stage, which can be transported from Europe to Asia.
本研究的目的是评估冷冻保存的欧洲鳗鱼精子和日本鳗鱼天然精子对早期受精、孵化、存活以及幼体畸形率的影响，并开发区分不同鳗鱼种类的分子技术。用日本鳗鱼雄性的精子和来自欧洲鳗鱼（A. anguilla，稀释液为改良的田中溶液，冷冻保护剂为甲醇）的冷冻保存精子对日本鳗鱼雌性（Anguilla japonica）的卵子进行人工授精。由于个体差异较大，在孵化后10天的受精、孵化和存活等测量参数之间没有统计学差异（p>0.05）。与孵化率相比，冷冻保存组幼体的畸形率高于对照组，这表明该方法需要进一步完善。遗传分析（PCR-RFLP、PCR-HRM）在检测日本鳗鱼和欧洲鳗鱼杂交中的父本贡献方面得到了明确结果，并且所应用的PCR-HRM方法是一种快速且经济高效的工具，可用于在玻璃鳗阶段识别非法进口的欧洲鳗鱼，这些玻璃鳗可从欧洲运输到亚洲。
