LC-ESI-MS/MS 同时定量分析马血浆中的游离姜黄素及其代谢物。
Simultaneous quantification of free curcuminoids and their metabolites in equine plasma by LC-ESI-MS/MS.
机构信息
Bioactive Botanical Research Laboratory, Department of Biomedical and Pharmaceutical Sciences, College of Pharmacy, University of Rhode Island, 7 Greenhouse Road, Kingston, RI, 02881, USA.
Gluck Equine Research Center, Department of Veterinary Science, University of Kentucky, 1400 Nicholasville Road, Lexington, KY, 40546, USA.
出版信息
J Pharm Biomed Anal. 2018 May 30;154:31-39. doi: 10.1016/j.jpba.2018.03.014. Epub 2018 Mar 7.
The human health benefits attributed to turmeric/curcumin spice has resulted in its wide utilization as a dietary supplement for companion pets and other animals including horses. While the quantification of free curcuminoids (curcumin, demethoxycurcumin, bisdemethoxycurcumin) and their phase-2 metabolites (curcumin-O-sulfate, curcumin-O-glucuronide) have been extensively investigated in human and rodent biological samples (primarily plasma and serum), there is lack of similar data for horses. Herein, we report a validated LC-ESI-MS/MS method for the simultaneous quantification of the aforementioned free curcuminoids and their metabolites in equine plasma. The linearity of the aforementioned curcuminoids and curcumin-O-sulfate was in the range of 0.5-1000 ng/mL and 1-1000 ng/mL for curcumin-O-glucuronide with 85-115% accuracy and <15% precision in equine plasma. The method was validated based on US FDA criteria and applied to characterize the pharmacokinetics of curcumin-O-sulfate in equine plasma.
姜黄/姜黄素香料对人类健康的益处使其被广泛用作伴侣宠物和其他动物（包括马）的膳食补充剂。虽然游离姜黄素类（姜黄素、脱甲氧基姜黄素、双脱甲氧基姜黄素）及其相 2 代谢物（姜黄素-O-硫酸盐、姜黄素-O-葡糖苷酸）在人类和啮齿动物生物样本（主要是血浆和血清）中的定量已有广泛研究，但马类动物的类似数据却缺乏。在此，我们报告了一种经验证的 LC-ESI-MS/MS 方法，用于同时定量马血浆中的上述游离姜黄素类和其代谢物。上述姜黄素类和姜黄素-O-硫酸盐的线性范围为 0.5-1000ng/mL 和 1-1000ng/mL，对于姜黄素-O-葡糖苷酸，其准确度为 85-115%，马血浆中的精密度<15%。该方法基于美国 FDA 标准进行验证，并应用于表征马血浆中姜黄素-O-硫酸盐的药代动力学。
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