Herebian Diran, Choi Jeong-Heui, Abd El-Aty A M, Shim Jae-Han, Spiteller Michael
Institute of Environmental Research, University of Dortmund, Dortmund, Germany.
Biomed Chromatogr. 2009 Sep;23(9):951-65. doi: 10.1002/bmc.1207.
The metabolic profile of polar (methanol) and non-polar (hexane) extracts of Curcuma domestica, a widely used medicinal plant, was established using various different analytical techniques, including GC-FID, GC-MS, HR-GC-MS and analytical HPLC-ESI-MS/MS by means of LTQ-Orbitrap technology. The major non-volatile curcuminoids curcumin, demethoxycurcumin and bisdemethoxycurcumin were identified when their chromatographic and precursor ion masses were compared with those of authentic standard compounds. In this paper we describe for the first time a GC/MS-based method for metabolic profiling of the hydrophilic extract. We also identified 61 polar metabolites as TMS derivatives.
利用包括气相色谱-火焰离子化检测器（GC-FID）、气相色谱-质谱联用仪（GC-MS）、高分辨气相色谱-质谱联用仪（HR-GC-MS）以及采用LTQ-Orbitrap技术的分析型高效液相色谱-电喷雾串联质谱（HPLC-ESI-MS/MS）等各种不同的分析技术，建立了广泛使用的药用植物姜黄的极性（甲醇）提取物和非极性（己烷）提取物的代谢谱。通过将其色谱图和前体离子质量与标准对照品的进行比较，鉴定出了主要的非挥发性姜黄素类化合物姜黄素、去甲氧基姜黄素和双去甲氧基姜黄素。在本文中，我们首次描述了一种基于GC/MS的亲水性提取物代谢谱分析方法。我们还鉴定出61种极性代谢物为三甲基硅烷（TMS）衍生物。
