School of Biotechnology and Health Sciences, Wuyi University; International Healthcare Innovation Institute (Jiangmen), Jiangmen 529020, China.
Bioactive Botanical Research Laboratory, Department of Biomedical and Pharmaceutical Sciences, College of Pharmacy, University of Rhode Island, Kingston, RI 02881, USA.
Nutrients. 2018 Aug 22;10(9):1139. doi: 10.3390/nu10091139.
(Mucuna) has been prescribed in Ayurveda for various brain ailments including 'kampavata' (tremors) or Parkinson's disease (PD). While Mucuna is a well-known natural source of levodopa (L-dopa), published studies suggest that other bioactive compounds may also be responsible for its anti-PD effects. To investigate this hypothesis, an L-dopa reduced (<0.1%) seeds extract (MPE) was prepared and evaluated for its anti-PD effects in cellular (murine BV-2 microglia and human SH-SY5Y neuroblastoma cells), , and models. In BV-2 cells, MPE (12.5⁻50 μg/mL) reduced hydrogen peroxide-induced cytotoxicity (15.7-18.6%), decreased reactive oxygen species production (29.1-61.6%), and lowered lipopolysaccharide (LPS)-induced nitric oxide species release by 8.9⁻60%. MPE (12.5-50 μg/mL) mitigated SH-SY5Y cell apoptosis by 6.9-40.0% in a non-contact co-culture assay with cell-free supernatants from LPS-treated BV-2 cells. MPE (12.5-50 μg/mL) reduced 6-hydroxydopamine (6-OHDA)-induced cell death of SH-SY5Y cells by 11.85⁻38.5%. Furthermore, MPE (12.5-50 μg/mL) increased median (25%) and maximum survival (47.8%) of exposed to the dopaminergic neurotoxin, methyl-4-phenylpyridinium. MPE (40 μg/mL) ameliorated dopaminergic neurotoxin (6-OHDA and rotenone) induced precipitation of innate negative geotaxis behavior of by 35.3 and 32.8%, respectively. Therefore, MPE contains bioactive compounds, beyond L-dopa, which may impart neuroprotective effects against PD.
（黎豆）已在阿育吠陀中被用于治疗各种脑部疾病，包括“坎帕瓦特”（震颤）或帕金森病（PD）。虽然黎豆是左旋多巴（L-dopa）的众所周知的天然来源，但已发表的研究表明，其他生物活性化合物也可能对其抗 PD 作用负责。为了验证这一假设，制备了一种左旋多巴还原（<0.1%）种子提取物（MPE），并在细胞（鼠 BV-2 小胶质细胞和人 SH-SY5Y 神经母细胞瘤细胞）、和体内模型中评估其抗 PD 作用。在 BV-2 细胞中，MPE（12.5⁻50μg/mL）降低了过氧化氢诱导的细胞毒性（15.7-18.6%），减少了活性氧的产生（29.1-61.6%），并降低了脂多糖（LPS）诱导的一氧化氮物质释放量达 8.9⁻60%。MPE（12.5-50μg/mL）在无细胞共培养测定中减轻了 LPS 处理的 BV-2 细胞的细胞培养液对 SH-SY5Y 细胞的凋亡作用，减轻程度为 6.9-40.0%。MPE（12.5-50μg/mL）降低了 6-羟基多巴胺（6-OHDA）诱导的 SH-SY5Y 细胞死亡，降低幅度为 11.85⁻38.5%。此外，MPE（12.5-50μg/mL）增加了暴露于多巴胺能神经毒素甲基-4-苯基吡啶的 SH-SY5Y 细胞的中位数（25%）和最大存活率（47.8%）。MPE（40μg/mL）分别改善了多巴胺能神经毒素（6-OHDA 和鱼藤酮）诱导的先天负趋地性行为的沉淀，改善幅度为 35.3%和 32.8%。因此，MPE 含有除 L-dopa 之外的生物活性化合物，可能对 PD 具有神经保护作用。
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