Dipartimento di Biologia, Università degli Studi di Napoli Federico II, via Cinthia 4, 80126, Napoli, Italy.
Dipartimento di Biologia, Università degli Studi di Napoli Federico II, via Cinthia 4, 80126, Napoli, Italy.
J Hazard Mater. 2018 Jun 5;351:131-137. doi: 10.1016/j.jhazmat.2018.02.044. Epub 2018 Mar 1.
In the present work the cytomorphological and physiological effects on three cardoon cultivars - Sardo, Siciliano, Spagnolo - grown in a metal-polluted soil, were investigated, to assess the traits concurring to the high tolerance to metal stress observed in cv. Spagnolo compared to the other two cultivars. The plants were grown for one month on a real polluted soil collected at a dismantling battery plant, highly enriched by heavy metals, especially Cd and Pb, and their leaves were analyzed by a multidisciplinary approach. TEM observations highlighted severe ultrastructural damage in Sardo and Siciliano, and preserved cytological traits in Spagnolo. Both pigment content and photochemistry indicated a decline in photosynthesis in Sardo and Sicilano and a substantial stability of the same parameters in Spagnolo. Protein analysis indicated a decrease in D1 level in all cultivars; in Spagnolo the D1 decrease was more pronounced and associated to a significant increase in Rubisco, a pattern likely preserving photosynthetic efficiency and high biomass production. In conclusion, Spagnolo cardoon was able to face metal stress through a prompt, multiple response balancing structural and functional traits.
本工作研究了在受金属污染的土壤中生长的三种刺菜品种（Sardo、Siciliano、Spagnolo）的细胞形态和生理效应，以评估与 Spagnolo 品种相比其他两个品种观察到的高耐金属胁迫特性相关的特征。这些植物在一个从一家废弃电池厂采集的富含重金属（尤其是 Cd 和 Pb）的真实污染土壤中生长了一个月，并用多学科方法对其叶片进行了分析。TEM 观察表明 Sardo 和 Siciliano 的超微结构受到严重损伤，而 Spagnolo 则保留了细胞学特征。色素含量和光化学均表明 Sardo 和 Siciliano 的光合作用下降，而 Spagnolo 则保持了这些参数的基本稳定性。蛋白质分析表明所有品种的 D1 水平均降低；在 Spagnolo 中，D1 的降低更为明显，并与 Rubisco 的显著增加相关，这种模式可能保持了较高的光合作用效率和生物量产量。总之，Spagnolo 刺菜通过迅速的、多重的反应平衡结构和功能特性来应对金属胁迫。
