• 文献检索
  • 文档翻译
  • 深度研究
  • 学术资讯
  • Suppr Zotero 插件Zotero 插件
  • 邀请有礼
  • 套餐&价格
  • 历史记录
应用&插件
Suppr Zotero 插件Zotero 插件浏览器插件Mac 客户端Windows 客户端微信小程序
定价
高级版会员购买积分包购买API积分包
服务
文献检索文档翻译深度研究API 文档MCP 服务
关于我们
关于 Suppr公司介绍联系我们用户协议隐私条款
关注我们

Suppr 超能文献

核心技术专利：CN118964589B侵权必究
粤ICP备2023148730 号-1Suppr @ 2026

文献检索

告别复杂PubMed语法，用中文像聊天一样搜索，搜遍4000万医学文献。AI智能推荐，让科研检索更轻松。

立即免费搜索

文件翻译

保留排版，准确专业，支持PDF/Word/PPT等文件格式，支持 12+语言互译。

免费翻译文档

深度研究

AI帮你快速写综述，25分钟生成高质量综述，智能提取关键信息，辅助科研写作。

立即免费体验

迈向基于刺菜蓟（变种）的生物炼制厂：通过苯丙烷途径的遗传调控改良细胞培养的案例研究。

Towards a Cardoon ( var. )-Based Biorefinery: A Case Study of Improved Cell Cultures via Genetic Modulation of the Phenylpropanoid Pathway.

机构信息

National Research Council-Institute of Agricultural Biology and Biotechnology (CNR-IBBA), Via Edoardo Bassini 15, 20133 Milano, Italy.

Department of Pharmacy-University of Naples Federico II (UNINA), Via Domenico Montesano 49, 80131 Naples, Italy.

出版信息

Int J Mol Sci. 2021 Nov 5;22(21):11978. doi: 10.3390/ijms222111978.

DOI:10.3390/ijms222111978
PMID:34769407
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC8584892/
Abstract

Cultivated cardoon ( var. L.) is a promising candidate species for the development of plant cell cultures suitable for large-scale biomass production and recovery of nutraceuticals. We set up a protocol for -mediated transformation, which can be used for the improvement of cardoon cell cultures in a frame of biorefinery. As high lignin content determines lower saccharification yields for the biomass, we opted for a biotechnological approach, with the purpose of reducing lignin content; we generated transgenic lines overexpressing the MYB4 transcription factor, a known repressor of lignin/flavonoid biosynthesis. Here, we report a comprehensive characterization, including metabolic and transcriptomic analyses of overexpression cardoon lines, in comparison to wild type, underlining favorable traits for their use in biorefinery. Among these, the improved accessibility of the lignocellulosic biomass to degrading enzymes due to depletion of lignin content, the unexpected increased growth rates, and the valuable nutraceutical profiles, in particular for hydroxycinnamic/caffeoylquinic and fatty acids profiles.

摘要

培育的刺菜蓟（ var. L.）是一种很有前途的候选物种，可用于开发适合大规模生物量生产和回收营养保健品的植物细胞培养物。我们建立了一种介导的转化方案，可用于在生物炼制框架内改善刺菜蓟细胞培养物。由于高木质素含量决定了生物质的糖化产率较低，我们选择了一种生物技术方法，目的是降低木质素含量；我们生成了过表达 MYB4 转录因子的转基因系，该转录因子是木质素/类黄酮生物合成的已知抑制剂。在这里，我们报告了对过表达刺菜蓟系的综合表征，包括代谢和转录组分析，并与野生型进行了比较，这些特性有利于它们在生物炼制中的应用。其中，由于木质素含量的降低导致木质纤维素生物质对降解酶的可及性提高，出乎意料的是生长速度加快，以及有价值的营养保健品谱，特别是羟基肉桂酸/咖啡酰奎宁酸和脂肪酸谱。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a634/8584892/03aeaa05e01f/ijms-22-11978-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a634/8584892/d8552597a3b4/ijms-22-11978-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a634/8584892/db25048caef3/ijms-22-11978-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a634/8584892/3e755f2d11f3/ijms-22-11978-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a634/8584892/fe08a596e4cc/ijms-22-11978-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a634/8584892/3c4e03e8ae09/ijms-22-11978-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a634/8584892/03aeaa05e01f/ijms-22-11978-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a634/8584892/d8552597a3b4/ijms-22-11978-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a634/8584892/db25048caef3/ijms-22-11978-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a634/8584892/3e755f2d11f3/ijms-22-11978-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a634/8584892/fe08a596e4cc/ijms-22-11978-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a634/8584892/3c4e03e8ae09/ijms-22-11978-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a634/8584892/03aeaa05e01f/ijms-22-11978-g006.jpg

相似文献

1
Towards a Cardoon ( var. )-Based Biorefinery: A Case Study of Improved Cell Cultures via Genetic Modulation of the Phenylpropanoid Pathway.迈向基于刺菜蓟（变种）的生物炼制厂：通过苯丙烷途径的遗传调控改良细胞培养的案例研究。
Int J Mol Sci. 2021 Nov 5;22(21):11978. doi: 10.3390/ijms222111978.
2
Enzymatic saccharification and bioethanol production from Cynara cardunculus pretreated by steam explosion.用蒸汽爆破预处理的蓟预处理酶糖化和生物乙醇生产。
Bioresour Technol. 2015 Jun;186:309-315. doi: 10.1016/j.biortech.2015.03.037. Epub 2015 Mar 14.
3
Development of a High Oleic Cardoon Cell Culture Platform by Overexpression and RNAi-Mediated Silencing.通过过表达和RNA干扰介导的沉默技术开发高油酸刺菜蓟细胞培养平台
Front Plant Sci. 2022 Jun 20;13:913374. doi: 10.3389/fpls.2022.913374. eCollection 2022.
4
Isolation and functional characterization of a cDNA coding a hydroxycinnamoyltransferase involved in phenylpropanoid biosynthesis in Cynara cardunculus L.编码参与菜蓟苯丙烷生物合成的羟基肉桂酰转移酶的cDNA的分离与功能鉴定
BMC Plant Biol. 2007 Mar 20;7:14. doi: 10.1186/1471-2229-7-14.
5
The rolC gene increases caffeoylquinic acid production in transformed artichoke cells.rolC基因可提高转化的洋蓟细胞中咖啡酰奎宁酸的产量。
Appl Microbiol Biotechnol. 2014 Sep;98(18):7773-80. doi: 10.1007/s00253-014-5869-2. Epub 2014 Jun 18.
6
Caffeoylquinic acids and flavonoids in the immature inflorescence of globe artichoke, wild cardoon, and cultivated cardoon.朝鲜蓟未成熟花序中的绿原酸和类黄酮，野生刺菜蓟和栽培刺菜蓟。
J Agric Food Chem. 2010 Jan 27;58(2):1026-31. doi: 10.1021/jf903311j.
7
Analysis of molecular genetic diversity of cardoon (Cynara cardunculus L.) in Tunisia.突尼斯刺菜蓟（Cynara cardunculus L.）的分子遗传多样性分析。
C R Biol. 2012 Jun;335(6):389-97. doi: 10.1016/j.crvi.2012.04.005. Epub 2012 May 25.
8
Cardoon as a Sustainable Crop for Biomass and Bioactive Compounds Production.蓟作为生物量和生物活性化合物生产的可持续作物。
Chem Biodivers. 2019 Dec;16(12):e1900498. doi: 10.1002/cbdv.201900498. Epub 2019 Nov 28.
9
Salinity source-induced changes in yield, mineral composition, phenolic acids and flavonoids in leaves of artichoke and cardoon grown in floating system.漂浮系统中生长的朝鲜蓟和洋蓟叶片的产量、矿物质组成、酚酸和类黄酮因盐度来源变化而变化。
J Sci Food Agric. 2014 Apr;94(6):1231-7. doi: 10.1002/jsfa.6403. Epub 2013 Oct 23.
10
Hybrid transcriptome sequencing approach improved assembly and gene annotation in Cynara cardunculus (L.).混合转录组测序方法改进了刺菜蓟（Cynara cardunculus (L.)）的组装和基因注释。
BMC Genomics. 2020 Aug 21;21(1):317. doi: 10.1186/s12864-020-6670-5.

引用本文的文献

1
Development of a High Oleic Cardoon Cell Culture Platform by Overexpression and RNAi-Mediated Silencing.通过过表达和RNA干扰介导的沉默技术开发高油酸刺菜蓟细胞培养平台
Front Plant Sci. 2022 Jun 20;13:913374. doi: 10.3389/fpls.2022.913374. eCollection 2022.
2
Abiotic Stresses Elicitation Potentiates the Productiveness of Cardoon Calli as Bio-Factories for Specialized Metabolites Production.非生物胁迫诱导增强了刺菜蓟愈伤组织作为特殊代谢产物生产生物工厂的生产力。
Antioxidants (Basel). 2022 May 24;11(6):1041. doi: 10.3390/antiox11061041.
3
Use of Different Organic Carbon Sources in Cells: Effects on Biomass Productivity and Secondary Metabolites.

本文引用的文献

1
A regulatory network driving shoot lignification in rapidly growing bamboo.调控网络驱动快速生长的竹子茎木质化。
Plant Physiol. 2021 Oct 5;187(2):900-916. doi: 10.1093/plphys/kiab289.
2
Recent Advanced Metabolic and Genetic Engineering of Phenylpropanoid Biosynthetic Pathways.近期苯丙烷类生物合成途径的代谢与遗传工程进展。
Int J Mol Sci. 2021 Sep 3;22(17):9544. doi: 10.3390/ijms22179544.
3
Increasing Monounsaturated Fatty Acid Contents in Hexaploid Seed Oil by Gene Knockout Using CRISPR-Cas9.利用CRISPR-Cas9基因敲除技术提高六倍体种子油中不饱和脂肪酸含量
细胞中不同有机碳源的使用：对生物量生产力和次生代谢产物的影响。
Plants (Basel). 2022 Mar 5;11(5):701. doi: 10.3390/plants11050701.
Front Plant Sci. 2021 Jun 29;12:702930. doi: 10.3389/fpls.2021.702930. eCollection 2021.
4
Strategies to Modulate Specialized Metabolism in Mediterranean Crops: From Molecular Aspects to Field.调控地中海作物特有代谢的策略：从分子层面到田间。
Int J Mol Sci. 2021 Mar 12;22(6):2887. doi: 10.3390/ijms22062887.
5
A conservative pathway for coordination of cell wall biosynthesis and cell cycle progression in plants.植物细胞壁生物合成与细胞周期进程协调的保守途径。
Plant J. 2021 May;106(3):630-648. doi: 10.1111/tpj.15187. Epub 2021 Mar 23.
6
Changes in Phenolics and Fatty Acids Composition and Related Gene Expression during the Development from Seed to Leaves of Three Cultivated Cardoon Genotypes.三种栽培刺菜蓟基因型从种子到叶片发育过程中酚类和脂肪酸组成及相关基因表达的变化
Antioxidants (Basel). 2020 Nov 8;9(11):1096. doi: 10.3390/antiox9111096.
7
AtMIF1 increases seed oil content by attenuating GL2 inhibition.AtMIF1 通过削弱 GL2 的抑制作用来增加种子油含量。
New Phytol. 2021 Feb;229(4):2152-2162. doi: 10.1111/nph.17016. Epub 2020 Nov 29.
8
Cellular, Molecular, and Physiological Aspects of In Vitro Plant Regeneration.植物离体再生的细胞、分子及生理学方面
Plants (Basel). 2020 Jun 1;9(6):702. doi: 10.3390/plants9060702.
9
A New Insight on Cardoon Exploring New Uses besides Cheese Making with a View to Zero Waste.刺菜蓟新见解：探索除奶酪制作之外的新用途以实现零浪费
Foods. 2020 May 2;9(5):564. doi: 10.3390/foods9050564.
10
Physiological, Biochemical, and Metabolic Responses to Short and Prolonged Saline Stress in Two Cultivated Cardoon Genotypes.两种栽培刺菜蓟基因型对短期和长期盐胁迫的生理、生化及代谢响应
Plants (Basel). 2020 Apr 27;9(5):554. doi: 10.3390/plants9050554.