School of Environmental and Chemical Engineering, Tianjin Polytechnic University, Tianjin, 300387, China; Stockbridge School of Agriculture, University of Masschusetts, Amherst, MA, 01003-9286, USA.
School of Environmental and Chemical Engineering, Tianjin Polytechnic University, Tianjin, 300387, China.
Environ Pollut. 2019 Jul;250:357-365. doi: 10.1016/j.envpol.2019.04.022. Epub 2019 Apr 11.
Herein, we investigated the effects of di-n-butyl phthalate (DBP) on photosynthesis, ribulose-1,5-bisphosphate carboxylase/oxygenase (RuBisCO) content, oxidative damage, and biomass accumulation of different tissues in wheat (Triticum aestivum L) planted in cinnamon soils. The photosynthetic or fluorescence parameters (except for the intercellular carbon dioxide concentration), chlorophyll content, RuBisCO content, and biomass of roots, stems, and leaves decreased at the seedling, jointing, and booting stages under the stress of DBP. Compared with the control, the content of superoxide anions and hydrogen peroxide in the roots, stems, and leaves increased with increasing DBP concentrations at the seedling, jointing, and booting stages. The activities of superoxide dismutase (SOD) and catalase (CAT) in the roots, stems, and leaves increased under the 10 and 20 mg kg DBP treatments; however, no significant changes were observed under the 40 mg kg DBP treatment at the seedling stage (except for the SOD activity in roots). The increase in SOD and CAT activities in the roots, stems, and leaves with increasing DBP concentration at the jointing and booting stages suggested that an increase in the activities of these antioxidant enzymes may play an important role in defending against excess reactive oxygen species under DBP stress. The biomass of wheat roots, stems, and leaves decreased with an increase in DBP concentration, which was presumably caused by a decrease in photosynthesis and RuBisCO. The effect of DBP on wheat roots, stems, and leaves decreased with wheat growth.
在此，我们研究了邻苯二甲酸二丁酯（DBP）对肉桂土中种植的小麦不同组织光合作用、核酮糖-1,5-二磷酸羧化酶/加氧酶（RuBisCO）含量、氧化损伤和生物量积累的影响。在 DBP 胁迫下，小麦幼苗、拔节期和孕穗期的光合或荧光参数（胞间二氧化碳浓度除外）、叶绿素含量、RuBisCO 含量和根、茎和叶的生物量均降低。与对照相比，在幼苗、拔节期和孕穗期，随着 DBP 浓度的增加，根、茎和叶中超氧阴离子和过氧化氢的含量增加。在 10 和 20mg/kg DBP 处理下，根、茎和叶中的超氧化物歧化酶（SOD）和过氧化氢酶（CAT）活性增加；然而，在幼苗期 40mg/kg DBP 处理下，这些酶活性没有显著变化（根中的 SOD 活性除外）。在拔节期和孕穗期，随着 DBP 浓度的增加，根、茎和叶中 SOD 和 CAT 活性的增加表明，这些抗氧化酶活性的增加可能在 DBP 胁迫下对过量活性氧的防御中发挥重要作用。随着 DBP 浓度的增加，小麦根、茎和叶的生物量减少，这可能是由于光合作用和 RuBisCO 减少所致。DBP 对小麦根、茎和叶的影响随小麦生长而降低。
