Department of Chemistry, National Sun Yat-sen University, Kaohsiung, 70, Lien-Hai Road, Kaohsiung, 80424, Taiwan.
Department of Materials and Optoelectronic Science, College of Engineering, National Sun Yat-sen University, Kaohsiung, 80424, Taiwan.
Anal Chim Acta. 2018 Jul 26;1015:58-65. doi: 10.1016/j.aca.2018.01.057. Epub 2018 Feb 3.
We report a rapid and facile method for detection of hydrogen peroxide (HO) in biological fluids using sub-stoichiometric two-dimensional (2D) molybdenum trioxide (α-MoO) nanoflakes. The two-dimensional nanoflakes, initially blue in color, is oxidized after interaction with hydrogen peroxide thereby changing its oxidation state to form α-MoO. The change in oxidation state of nanoflakes transforms from blue to a visually distinct hazy blue color with change in absorption spectrum. The phenomenal property is explored here in sensing up to 34 nM as limit of detection. The efficacy of the detection system was analyzed by "zone of inhibition" based agar diffusion assay with different concentrations of HO. The current approach is highly accurate, effective and reproducible for quantification of physiological concentration of HO in biological fluid such as human urine.
我们报告了一种使用亚化学计量二维（2D）三氧化钼（α-MoO）纳米薄片快速简便地检测生物流体中过氧化氢（HO）的方法。最初呈蓝色的二维纳米薄片与过氧化氢相互作用后被氧化，从而改变其氧化态形成α-MoO。纳米薄片的氧化态变化从蓝色变为视觉上明显的朦胧蓝色，同时吸收光谱发生变化。在这里，我们探索了这种现象特性，可检测低至 34 nM 的 HO，检测限为 34 nM。基于 HO 不同浓度的琼脂扩散分析，分析了检测系统的功效。该方法对于定量分析生物流体（如人尿）中生理浓度的 HO 非常准确、有效且重现性好。
