使用苯并硼氧六环水凝胶的糖类染料置换测定法。
Dye Displacement Assay for Saccharides using Benzoxaborole Hydrogels.
作者信息
Lampard Emma V, Sedgwick Adam C, Sombuttan Thitima, Williams George T, Wannalerse Boontana, Jenkins A Toby A, Bull Steven D, James Tony D
机构信息
Department of Chemistry University of Bath BA2 7AY Bath UK.
The Department of Chemistry, Faculty of Science Kasetsart University 50 Ngam Wong Wan Road, Lat Yao, Chatuchak Bangkok 10900 Thailand.
出版信息
ChemistryOpen. 2018 Jan 30;7(3):266-268. doi: 10.1002/open.201700193. eCollection 2018 Mar.
Dye displacement assays are a simple but effective method to determine the concentration of target analytes. Previously, we have shown that phenylboronic acid pinacol ester hydrogels (borogels) can be used to develop a boronic acid-Alizarin red S dye displacement assay for the determination of fructose (orange to red). In this work, benzoxaborole hydrogels (BOBgels) were developed, and these BOBgels demonstrated an enhanced apparent binding affinity towards monosaccharides, in particular towards glucose.
染料置换分析法是一种简单但有效的测定目标分析物浓度的方法。此前，我们已表明苯硼酸频哪醇酯水凝胶（硼凝胶）可用于开发一种硼酸 - 茜素红S染料置换分析法来测定果糖（由橙色变为红色）。在这项工作中，开发了苯并硼唑水凝胶（BOB凝胶），并且这些BOB凝胶对单糖，特别是对葡萄糖表现出增强的表观结合亲和力。
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