Medical Mycology Group, Microbiology and Parasitology Department, School of Medicine, Universidad de Antioquia, Medellín, Colombia.
Cellular and Molecular Biology Group, Corporación para Investigaciones Biológicas (CIB), Universidad de Antioquia, Medellín, Colombia.
Am J Trop Med Hyg. 2018 May;98(5):1303-1312. doi: 10.4269/ajtmh.17-0214. Epub 2018 Mar 8.
is the causative agent of histoplasmosis and this fungus inhabits soils rich in phosphorus and nitrogen that are enriched with bird and bat manure. The replacement of organic matter in agroecosystems is necessary in the tropics, and the use of organic fertilizers has increased. Cases and outbreaks due to the presence of the fungus in these components have been reported. The Instituto Colombiano Agropecuario resolution 150 of 2003 contains the parameters set by the Colombian Technical Standard (NTC 5167) on the physicochemical and microbiological features of fertilizers, but it does not regulate the search for . The aim of this study was to demonstrate presence in organic fertilizers by nested polymerase chain reaction (PCR). A total of 239 samples were collected: 201 (84.1%) corresponded to organic fertilizers, 30 (12.5%) to bird excrement, and 8 (3.4%) to cave soils. The Hc100 nested PCR had a detection limit of 0.1 pg/µL and a specificity of 100%. A total of 25 (10.5%) samples were positive and validated by sequencing. Seven of the positive samples represented locations where was previously detected, suggesting the persistence of the fungus. No significant correlations were detected between the physicochemical and microbiological parameters with the presence of by nested PCR, indicating the fungus existence in organic fertilizers that complied with the NTC 5167. The Hc100 nested PCR targeting standardized in this work will improve the evaluation of organic fertilizers and ensure the prevention of outbreaks and cases due to manufacturing, marketing, and use of fertilizers contaminated with .
组织胞浆菌是组织胞浆菌病的病原体，这种真菌栖息在富含磷和氮的土壤中，这些土壤富含鸟类和蝙蝠的粪便。在热带地区，农业生态系统中需要更换有机物，有机肥的使用量有所增加。由于这些成分中存在真菌，已经报告了一些病例和爆发。2003 年哥伦比亚农业研究所第 150 号决议包含了哥伦比亚技术标准（NTC 5167）规定的肥料物理化学和微生物特性的参数，但没有规定对 的搜索。本研究旨在通过巢式聚合酶链反应（PCR）证明有机肥中存在 。共采集了 239 个样本：201 个（84.1%）为有机肥，30 个（12.5%）为鸟粪，8 个（3.4%）为洞穴土壤。Hc100 巢式 PCR 的检测限为 0.1 pg/µL，特异性为 100%。共有 25 个（10.5%）样本为阳性，并通过测序进行了验证。7 个阳性样本代表了先前检测到 的地点，表明该真菌的持续存在。巢式 PCR 检测到的 与理化和微生物参数之间未检测到显著相关性，表明该真菌存在于符合 NTC 5167 的有机肥中。本工作中针对 标准化的 Hc100 巢式 PCR 将提高对有机肥的评估，并确保因制造、销售和使用受污染的肥料而导致的爆发和病例得到预防。
