Department of Microbiology, Faculty of Medicine, Chiang Mai University, Chiang Mai, 50200, Thailand.
Mycopathologia. 2013 Dec;176(5-6):395-402. doi: 10.1007/s11046-013-9701-9. Epub 2013 Sep 13.
Histoplasmosis is a systemic mycosis caused by inhaling spores of Histoplasma capsulatum, a dimorphic fungus. This fungus grows in soil contaminated with bat and avian excreta. Each year, patients with disseminated histoplasmosis have been diagnosed in Chiang Mai, northern Thailand. No published information is currently available on the environmental sources of this fungus in Chiang Mai or anywhere else in Thailand. The aim of this study was to detect H. capsulatum in soil samples contaminated with bat guano and avian droppings by nested PCR. Two hundred and sixty-five samples were collected from the following three sources: soil contaminated with bat guano, 88 samples; soil contaminated with bird droppings, 86 samples; and soil contaminated with chicken droppings, 91 samples. Genomic DNA was directly extracted from each sample, and H. capsulatum was detected by nested PCR using a primer set specific to a gene encoding 100-kDa-like protein (HcI, HcII and HcIII, HcIV). Histoplasma capsulatum was detected in seven of 88 soil samples contaminated with bat guano, one of 21 soil samples contaminated with pigeon droppings and 10 of 91 soil samples contaminated with chicken droppings. The results indicate the possibility of the association of bat guano and chicken droppings with H. capsulatum in this area of Thailand.
组织胞浆菌病是一种系统性真菌病，由吸入荚膜组织胞浆菌的孢子引起，荚膜组织胞浆菌是一种二态真菌。这种真菌生长在受蝙蝠和禽鸟排泄物污染的土壤中。每年，在泰国北部清迈都会诊断出患有播散性组织胞浆菌病的患者。目前，尚无关于清迈或泰国其他任何地方该真菌的环境来源的公开信息。本研究旨在通过巢式 PCR 检测受蝙蝠粪便和禽鸟粪便污染的土壤样本中的荚膜组织胞浆菌。从以下三个来源采集了 265 个样本：受蝙蝠粪便污染的土壤样本 88 个；受禽鸟粪便污染的土壤样本 86 个；受鸡粪污染的土壤样本 91 个。直接从每个样本中提取基因组 DNA，并使用针对编码 100-kDa 样蛋白（HcI、HcII 和 HcIII、HcIV）的基因的引物组通过巢式 PCR 检测荚膜组织胞浆菌。在 88 个受蝙蝠粪便污染的土壤样本中有 7 个、在 21 个受鸽粪污染的土壤样本中有 1 个和在 91 个受鸡粪污染的土壤样本中有 10 个检测到荚膜组织胞浆菌。结果表明，在泰国的这一地区，蝙蝠粪便和鸡粪与荚膜组织胞浆菌之间存在关联的可能性。
