微小 RNA-130a 通过 PTEN/PI3K/Akt 信号通路调控软骨细胞增殖并缓解骨关节炎。
MicroRNA-130a regulates chondrocyte proliferation and alleviates osteoarthritis through PTEN/PI3K/Akt signaling pathway.
机构信息
Department of Orthopaedics, Shanghai Tongji Hospital, Shanghai 200065, P.R. China.
Shanghai Jiading Nanxiang Hospital, Shanghai 200065, P.R. China.
出版信息
Int J Mol Med. 2018 Jun;41(6):3699-3708. doi: 10.3892/ijmm.2018.3551. Epub 2018 Mar 9.
The function of microRNA‑130a in development and progression of osteoarthritis was determined. In osteoarthritis patients, the serum levels of microRNA‑130a were decreased, compared with normal group. Overexpression of microRNA‑130a increased cell proliferation and decreased apoptosis of chondrocytes, and downregulation of microRNA‑130a also decreased cell proliferation and induced apoptosis in chondrocytes. Downregulation of microRNA‑130a promoted Bax and caspase‑3/9 protein expression, increased inflammation divisors and suppressed the PTEN/PI3K/Akt signaling pathway. PTEN inhibitor, VO‑Ohpic trihydrate increased the destructive effect of microRNA‑130a on cell proliferation of chondrocytes. PI3K inhibitor, wortmannin also increased the destructive effect of microRNA‑130a on osteoarthritis. In conclusion, microRNA‑130a is an important regulator of osteoarthritis in chondrocytes through PTEN/PI3K/Akt signaling pathway.
研究了 microRNA-130a 在骨关节炎发生和发展中的作用。与正常组相比，骨关节炎患者血清中 microRNA-130a 的水平降低。microRNA-130a 的过表达增加了软骨细胞的增殖并减少了细胞凋亡，而 microRNA-130a 的下调也减少了软骨细胞的增殖并诱导了细胞凋亡。下调 microRNA-130a 促进 Bax 和 caspase-3/9 蛋白的表达，增加炎症因子并抑制了 PTEN/PI3K/Akt 信号通路。PTEN 抑制剂 VO-Ohpic 三水合物增加了 microRNA-130a 对软骨细胞增殖的破坏作用。PI3K 抑制剂 wortmannin 也增加了 microRNA-130a 对骨关节炎的破坏作用。总之，microRNA-130a 通过 PTEN/PI3K/Akt 信号通路成为软骨细胞中骨关节炎的重要调节因子。
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