Tseng Min, Chiang Wan-Ping, Liao Hsuen-Chun, Hsieh Sung-Yuan, Yuan Gao-Fung
Bioresource Collection and Research Center, Food Industry Research and Development Institute, HsinChu, 300, Taiwan, ROC.
Int J Syst Evol Microbiol. 2018 May;68(5):1418-1422. doi: 10.1099/ijsem.0.002653. Epub 2018 Mar 13.
Strain 06168H-1 was isolated from a fishpond sediment sample collected from the southern area of Taiwan, and a polyphasic approach was used to determine its taxonomic position. The isolate grew between 20-40 °C and 0-8 % (w/v) NaCl. It produced branched and unfragmented substrate mycelia. Short spore chains (3-10 spores per chain) formed on branched aerial mycelia. The spore chains contained non-motile, smooth-surfaced, oval spores. Galactose, arabinose and ribose were the whole-cell sugars and meso-diaminopimelic acid was present in its peptidoglycan. The polar lipids were diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine, phosphatidylmethylethanolamine, phosphatidylcholine, hydroxyphosphati dylethanolamine and a ninhydrin-positive phosphoglycolipid. The predominant menaquinone was MK-9(H4) and minor components were MK-8(H4) and MK-9(H6). Mycolic acids were not detected. The major cellular fatty acids were iso-C16 : 0 and C17 : 1ω6c and C17 : 0ω8c. The DNA G+C content of the strain was 70.6 mol%. Phylogenetic analysis based on 16S rRNA gene sequences showed this strain clustered with the members of the genus Saccharomonospora and was closely related to Saccharomonospora xinjiangensis, Saccharomonospora azurea and Saccharomonosporacyanea. The levels of similarity between this strain and the closely related species were: Sxinjiangensis BCRC16887, 98.34 %; S. azurea BCRC 16220, 98.27 %; and S. cyanea BCRC 16886, 97.99 %. Based on phylogenetic characteristics, strain 06168H-1 represents a novel species of the genus Saccharomonospora. We thus propose the name Saccharomonospora piscinae sp. nov. for this novel strain, with strain 06168H-1 (=BCRC 16893=KCTC 19743) as the type strain.
菌株06168H-1是从台湾南部地区采集的鱼塘沉积物样本中分离得到的，采用多相分类法确定其分类地位。该菌株在20-40°C和0-8%（w/v）NaCl条件下生长。它产生分支且不断裂的基内菌丝。在分支的气生菌丝上形成短孢子链（每条链3-10个孢子）。孢子链包含不运动、表面光滑的椭圆形孢子。半乳糖、阿拉伯糖和核糖是全细胞糖，其肽聚糖中存在内消旋二氨基庚二酸。极性脂质为双磷脂酰甘油、磷脂酰甘油、磷脂酰乙醇胺、磷脂酰甲基乙醇胺、磷脂酰胆碱、羟基磷脂酰乙醇胺和一种茚三酮阳性磷酸糖脂。主要的甲基萘醌是MK-9(H4)，次要成分是MK-8(H4)和MK-9(H6)。未检测到分枝菌酸。主要的细胞脂肪酸是异-C16 : 0和C17 : 1ω6c以及C17 : 0ω8c。该菌株的DNA G+C含量为70.6 mol%。基于16S rRNA基因序列的系统发育分析表明，该菌株与糖单孢菌属的成员聚类，并且与新疆糖单孢菌、天蓝糖单孢菌和蓝糖单孢菌密切相关。该菌株与密切相关物种的相似性水平为：新疆糖单孢菌BCRC16887，98.34%；天蓝糖单孢菌BCRC 16220，98.27%；蓝糖单孢菌BCRC 16886，97.99%。基于系统发育特征，菌株06168H-1代表糖单孢菌属的一个新物种。因此，我们为这个新菌株提议命名为嗜鱼糖单孢菌（Saccharomonospora piscinae sp. nov.），以菌株06168H-1（=BCRC 16893=KCTC 19743）作为模式菌株。
