Department of Pharmacy, School of Pharmaceutical Sciences, National Yang Ming Chiao Tung University (NYCU), Taipei 11221, Taiwan.
Department of Medical Research, China Medical University Hospital, China Medical University, Taichung 404, Taiwan.
Molecules. 2022 Mar 14;27(6):1887. doi: 10.3390/molecules27061887.
Bioassay-guided fractionation of extracts derived from solid cultures of a Herbidospora daliensis originating from Taiwan led to the isolation of five new compounds, for which we propose the name herbidosporadalins A−E (1−5), one isolated for the first time, herbidosporadalin F (6), together with two known compounds (7 & 8). Their structures were elucidated by spectroscopic analyses, including 1D- and 2D-NMR experiments with those of known analogues, and on the basis of HR-EI-MS mass spectrometry, their anti-inflammatory activities were also evaluated. Of these isolates, herbidosporadalin A (1), B (2), F (6) and G (8) showed NO inhibitory activity, with IC50 values of 11.8 ± 0.9, 7.1 ± 2.9, 17.8 ± 1.7, and 13.3 ± 6.5 μM, stronger than the positive control quercetin (IC50 = 36.8 ± 1.3 μM). To the best of our knowledge, this is the first report on 3,4-seco-friedelane metabolites (5, 6 & 8) from the genus Herbidospora.
从台湾产 Herbidospora daliensis 的固体培养物中提取的生物测定指导分离，得到了五个新化合物，我们将其命名为 herbidosporadalins A-E（1-5），其中一个是首次分离得到的，还有 herbidosporadalin F（6），以及两个已知化合物（7 和 8）。通过光谱分析，包括与已知类似物的 1D 和 2D-NMR 实验，以及基于高分辨率 EI-MS 质谱，确定了它们的结构，并评估了它们的抗炎活性。在这些分离物中，herbidospordalin A（1）、B（2）、F（6）和 G（8）对 NO 没有抑制活性，IC50 值分别为 11.8±0.9、7.1±2.9、17.8±1.7 和 13.3±6.5 μM，强于阳性对照槲皮素（IC50=36.8±1.3 μM）。据我们所知，这是首次从 Herbidospora 属中报道 3,4-裂夫烷代谢物（5、6 和 8）。
