Garcia Ribeiro Rita Sofia, Ketkar-Atre Ashwini, Yin Ting, Louchami Karim, Struys Tom, Lambrichts Ivo, Sener Abdullah, Malaisse Willy Jean, De Cuyper Marcel, Himmelreich Uwe
Biomedical MRI/MoSAIC, Department of Imaging and Pathology, University of Leuven, Herestraat 49, B3000 Leuven, Belgium.
Laboratory for Functional and Metabolic Imaging, Ecole Polytechnique Federale Lausanne, 1015 Lausanne, Switzerland.
J Pers Med. 2018 Mar 12;8(1):12. doi: 10.3390/jpm8010012.
Pancreatic islets (PIs) transplantation is an alternative approach for the treatment of severe forms of type 1 diabetes (T1D). To monitor the success of transplantation, it is desirable to follow the location of engrafted PIs non-invasively. In vivo magnetic resonance imaging (MRI) of transplanted PIs is a feasible cell tracking method; however, this requires labeling with a suitable contrast agent prior to transplantation. We have tested the feasibility of cationic magnetoliposomes (MLs), compared to commercial contrast agents (Endorem and Resovist), by labeling insulinoma cells and freshly isolated rat PIs. It was possible to incorporate Magnetic Ressonance (MR)-detectable amounts of MLs in a shorter time (4 h) when compared to Endorem and Resovist. MLs did not show negative effects on the PIs' viability and functional parameters in vitro. Labeled islets were transplanted in the renal sub-capsular region of healthy mice. Hypointense contrast in MR images due to the labeled PIs was detected in vivo upon transplantation, while MR detection of PIs labeled with Endorem and Resovist was only possible after the addition of transfection agents. These findings indicate that MLs are suitable to image PIs, without affecting their function, which is promising for future longitudinal pre-clinical and clinical studies involving the assessment of PI transplantation.
胰岛移植是治疗严重1型糖尿病（T1D）的一种替代方法。为监测移植的成功与否，期望以非侵入性方式追踪植入胰岛的位置。移植胰岛的体内磁共振成像（MRI）是一种可行的细胞追踪方法；然而，这需要在移植前用合适的造影剂进行标记。我们通过标记胰岛素瘤细胞和新鲜分离的大鼠胰岛，测试了阳离子磁性脂质体（MLs）与商用造影剂（Endorem和Resovist）相比的可行性。与Endorem和Resovist相比，在更短时间（4小时）内就有可能掺入可通过磁共振（MR）检测到的MLs量。MLs在体外对胰岛的活力和功能参数未显示出负面影响。将标记的胰岛移植到健康小鼠的肾被膜下区域。移植后在体内检测到由于标记胰岛导致的MR图像低信号造影，而用Endorem和Resovist标记的胰岛只有在添加转染剂后才能进行MR检测。这些发现表明，MLs适合对胰岛进行成像，且不影响其功能，这对于未来涉及评估胰岛移植的纵向临床前和临床研究很有前景。
