Reproductive Medical Center, The Sixth Affiliated Hospital of Sun Yat-sen University, 17th Shou-gou-ling Rd, Guangzhou, 510655, China.
Infertility Center of St. Louis, 224 S. Woods Mill Road Suite 730, St. Louis, MO, 63017, USA.
J Assist Reprod Genet. 2019 Jun;36(6):1225-1235. doi: 10.1007/s10815-019-01446-5. Epub 2019 Apr 13.
To evaluate the relationship between apoptosis of granulosa cells in women with normal ovarian reserve versus diminished ovarian reserve, and relate that to follicular fluid hormones, and to clinical outcomes.
A prospective cohort study was initiated between October 2015 and June 2016 involving a total of 164 women undergoing IVF/ICSI cycles at a single IVF center. Mural and cumulus granulosa cells, and follicularfluid were collected during oocyte retrieval. Annexin V-FITC/PI apoptosis staining and flow cytometryanalysis were performed to evaluate apoptosis rate of mural granulosa cells and cumulus cells. Follicularfluid hormones were measured by ECLIA. Laboratory and clinical outcomes were analyzed.
In mural granulosa cells, early, late and total apoptosis rates were significantly increased in women with diminished ovarian reserve when compare to women with normal ovarian reserve, along with lower AMHand progesterone levels (but higher estradiol levels) in follicular fluid. Early apoptosis rate of cumulus cellswas significantly higher in the non-pregnant group. The apoptosis rate of mural cells was negativelycorrelated with parameters related to ovarian response, oocyte yield, MII egg number, 2pn cleavagenumber, D3 good embryos number, blastocyst formation rate and frozen embryos number. A positivecorrelation was found between mural granulosa cell apoptosis and age.
A significantly higher apoptosis rate of mural granulosa cells was correlated with worse ovarian response, with fewer egg and embryo numbers in IVF/ICSI, as well as with age. Early apoptosis rate of cumulus cellsmight also have influence on clinical pregnancy.
评估卵巢储备正常与储备功能降低的妇女的颗粒细胞凋亡与卵泡液激素之间的关系，并将其与临床结局相关联。
这是一项前瞻性队列研究，于 2015 年 10 月至 2016 年 6 月在一家体外受精/卵胞浆内单精子注射（ICSI）中心进行，共纳入 164 名接受 IVF/ICSI 周期的妇女。在取卵过程中收集壁层和卵丘颗粒细胞及卵泡液。采用 Annexin V-FITC/PI 凋亡染色和流式细胞术分析评估壁层颗粒细胞和卵丘细胞的凋亡率。采用电化学发光免疫分析法（ECLIA）检测卵泡液激素。分析实验室和临床结局。
与卵巢储备正常的妇女相比，卵巢储备功能降低的妇女壁层颗粒细胞的早期、晚期和总凋亡率显著增加，同时卵泡液中的 AMH 和孕激素水平较低（但雌二醇水平较高）。非妊娠组卵丘细胞的早期凋亡率显著升高。壁层细胞的凋亡率与卵巢反应、获卵数、MII 卵数、2pn 分裂数、D3 优质胚胎数、囊胚形成率和冷冻胚胎数等与卵巢反应相关的参数呈负相关。壁层颗粒细胞凋亡与年龄呈正相关。
壁层颗粒细胞凋亡率显著升高与卵巢反应较差、IVF/ICSI 中卵母细胞和胚胎数量较少以及年龄有关。卵丘细胞的早期凋亡率可能也对临床妊娠有影响。
