Broad Institute of MIT and Harvard , 75 Ames Street , Cambridge , Massachusetts 02142 , United States.
Center for Systems Biology, Department of Organismic and Evolutionary Biology , Harvard University , Cambridge , Massachusetts 02138 , United States.
Anal Chem. 2018 Apr 3;90(7):4657-4662. doi: 10.1021/acs.analchem.7b05200. Epub 2018 Mar 21.
In blood samples from patients with viral infection, it is often important to separate viral particles from human cells, for example, to minimize background in performing viral whole genome sequencing. Here, we present a microfluidic device that uses spiral inertial microfluidics with continuous circulation to separate host cells from viral particles and free nucleic acid. We demonstrate that this device effectively reduces white blood cells, red blood cells, and platelets from both whole blood and plasma samples with excellent recovery of viral nucleic acid. Furthermore, microfluidic separation leads to greater viral genome coverage and depth, highlighting an important application of this device in processing clinical samples for viral genome sequencing.
在病毒感染患者的血液样本中，通常需要将病毒颗粒与人细胞分离，例如，尽量减少病毒全基因组测序中的背景干扰。在此，我们展示了一种使用螺旋惯性微流控技术并实现连续循环的微流控装置，用于分离宿主细胞和病毒颗粒及游离核酸。我们证明该装置可有效地从全血和血浆样本中去除白细胞、红细胞和血小板，同时病毒核酸的回收率良好。此外，微流控分离可提高病毒基因组的覆盖度和深度，突出了该装置在处理临床样本进行病毒基因组测序方面的重要应用。
