Çamlıca Yusuf, Bediz Salih Cüfer, Çömelekoğlu Ülkü, Yilmaz Şakir Necat
a Department of Biology , Mersin University , Mersin , Turkey.
b Department of Biophysics , Mersin University , Mersin , Turkey.
Drug Chem Toxicol. 2019 May;42(3):264-269. doi: 10.1080/01480545.2018.1442475. Epub 2018 Mar 14.
In this study, the effects of a neonicotinoid insecticide acetamiprid on the sciatic nerve of Rana ridibunda were investigated by using electrophysiological and histological methods. A total of 35 preparations of sciatic nerve isolated from 35 frogs (Nervus ischiadicus) were used in the experiments. Experiments were designed as four different dose groups (n = 8 per group). Acetamiprid solutions of 1 (group 1), 10 (group 2), 100 (group 3), and 1000 µM (group 4) were applied to the nerves in dose groups. In each group, action potentials were recorded before application of acetamiprid which served as control data. The extracellular action potentials were recorded for each group of 30th, 60th, 90th and 120th min of application time. Action potential amplitude and area were measured from recordings. Histological evaluation was performed by transmission electron microscopy. In electrophysiological examination, all doses in which acetamiprid applied have shown the effect from the 30th min and suppressed the sciatic nerve action potential. Acetamiprid significantly reduced the amplitude at the rate of 78-96% and the area at the rate of 79-98% (p < 0.05). In electron microscopic examination, the control nerves were in normal appearance. Disorganization, irregularity, dense ovoid body formation, fragmentation of the myelin sheath, and loss on some axoplasm of the nerves in the dose group have been observed. Our findings showed that acetamiprid can cause neuropathic changes in sciatic nerve at all applied doses. These results indicate that acetamiprid as other insecticides can have harmful effects on non-target organisms.
在本研究中，采用电生理和组织学方法研究了新烟碱类杀虫剂啶虫脒对泽蛙坐骨神经的影响。实验共使用了从35只青蛙分离出的35条坐骨神经标本。实验设计为四个不同剂量组（每组n = 8）。剂量组分别将1（第1组）、10（第2组）、100（第3组）和1000 μM（第4组）的啶虫脒溶液应用于神经。每组在应用啶虫脒之前记录动作电位，作为对照数据。在应用时间的第30、60、90和120分钟记录每组的细胞外动作电位。从记录中测量动作电位的幅度和面积。通过透射电子显微镜进行组织学评估。在电生理检查中，所有应用啶虫脒的剂量在第30分钟时均显示出作用，并抑制了坐骨神经动作电位。啶虫脒显著降低幅度78 - 96%，降低面积79 - 98%（p < 0.05）。在电子显微镜检查中，对照神经外观正常。在剂量组中观察到神经髓鞘排列紊乱、不规则、形成密集的卵圆形体、髓鞘断裂以及一些轴质丢失。我们的研究结果表明，所有应用剂量的啶虫脒均可导致坐骨神经发生神经病变。这些结果表明，啶虫脒与其他杀虫剂一样，可能对非靶标生物产生有害影响。
