Choi Ho-Jung, Kim Yeon-Hee
Biomedical Engineering and Biotechnology Major, Divison of Applied Bioengineering, Dong-Eui University, Busan 47340, Republic of Korea.
J Microbiol Biotechnol. 2018 May 28;28(5):826-830. doi: 10.4014/jmb.1802.02004.
A Cre/-δ-integration system was developed to allow sequential and simultaneous integration of a multiple gene expression cassette in . To allow repeated integrations, the reusable () carrying sequences was used, and the integrated was efficiently removed by inducing Cre recombinase. The and genes encoding endoxylanase and β-xylosidase, respectively, were used as model genes for xylan metabolism in this system, and the copy number of these genes was increased to 15.8 and 16.9 copies/cell, respectively, by repeated integration. This integration system is a promising approach for the easy construction of yeast strains with enhanced metabolic pathways through multicopy gene expression.
开发了一种Cre/loxP整合系统，以允许在……中顺序和同时整合多个基因表达盒。为了实现重复整合，使用了携带loxP序列的可重复使用的载体，并且通过诱导Cre重组酶有效地去除了整合的载体。分别编码内切木聚糖酶和β-木糖苷酶的xyn10A和xyl3基因被用作该系统中木聚糖代谢的模型基因，通过重复整合，这些基因的拷贝数分别增加到每个细胞15.8和16.9个拷贝。这种整合系统是一种很有前景的方法，可通过多拷贝基因表达轻松构建具有增强代谢途径的酵母菌株。
