Wu Renzhi, Chen Dong, Cao Shuwei, Lu Zhilong, Huang Jun, Lu Qi, Chen Ying, Chen Xiaoling, Guan Ni, Wei Yutuo, Huang Ribo
State Key Laboratory for Conservation and Utilization of Subtropical Agro-bioresources, College of Life Science and Technology, Guangxi University 100 Daxue Road Nanning Guangxi 530004 China
National Engineering Research Center for Non-food Biorefinery, State Key Laboratory of Non-food Biomass Enzyme Technology, Guangxi Key Laboratory of Biorefinery, Guangxi Academy of Sciences 98 Daling Road Nanning Guangxi 530007 China.
RSC Adv. 2020 Jan 10;10(4):2267-2276. doi: 10.1039/c9ra08673k. eCollection 2020 Jan 8.
Replacement of a novel candidate ethanol fermentation-associated regulatory gene, , from a fast-growing strain MC15, as determined through comparative genomics analysis among three yeast strains with significant differences in ethanol yield, is hypothesised to shorten the fermentation time and enhance ethanol production from sugarcane molasses. This study sought to test this hypothesis through a novel strategy involving the transfer of the gene from a low ethanol-producing, yet fast-growing strain MC15 to a high ethanol-producing industrial strain MF01 through homologous recombination. The results indicated that in the industrially engineered strain MF01-PHO4 displayed genomic stability with a mean maximum ethanol yield that rose to 114.71 g L, accounting for a 5.30% increase in ethanol yield and 12.5% decrease in fermentation time in comparison with that in the original strain MF01, which was the current highest ethanol-producing strain in SCM fermentation in the reported literature. These results serve to advance our current understanding of the association between improving ethanol yield and replacement of , while providing a feasible strategy for industrially engineered yeast strains to improve ethanol production efficiently.
通过对三株乙醇产量差异显著的酵母菌株进行比较基因组学分析，确定从快速生长菌株MC15中替换一个新的候选乙醇发酵相关调控基因，推测这将缩短发酵时间并提高甘蔗 molasses 的乙醇产量。本研究试图通过一种新策略来验证这一假设，该策略涉及通过同源重组将该基因从低乙醇产量但快速生长的菌株MC15转移到高乙醇产量的工业菌株MF01中。结果表明，工业工程菌株MF01 - PHO4中的该基因显示出基因组稳定性，平均最大乙醇产量升至114.71 g/L，与原始菌株MF01相比，乙醇产量增加了5.30%，发酵时间减少了12.5%，MF01是已报道文献中目前SCM发酵中乙醇产量最高的菌株。这些结果有助于推进我们目前对提高乙醇产量与该基因替换之间关联的理解，同时为工业工程酵母菌株高效提高乙醇产量提供了一种可行策略。
