Peng A, Xu L Q
Sci Total Environ. 1987 Jun;64(1-2):89-98. doi: 10.1016/0048-9697(87)90124-0.
To shed light on the causes of Kaschin-Beck disease, which can be prevented by supplementation of the diet with sodium selenite, the interactions between inorganic selenium compounds (selenite and selenate) and humic/fulvic acid were investigated. Selenate was found to be slowly reduced to selenite by humic acid in acidic solution. Selenite was adsorbed on manganese dioxide and iron(III) oxide from solution to a much greater degree than on kaolin, humic acid, Yongshu soil, or silicon dioxide. Feeding mice a diet supplemented with sodium selenite increased the selenium concentration in the kidney, liver, spleen, lung, heart and blood. A diet containing sodium selenite and fulvic acid caused the selenium concentrations in the organs, with the exception of the spleen, to be lower than observed with the selenite-only diet. Selenium and fulvic acid increase the activity of glutathione peroxidase. Sodium selenite and fulvic acid injected in combination into the abdominal cavity of mice and rats were less toxic than either substance alone. Selenite and fulvic acid applied separately enhanced the luminosity of photobacterium phosphoreum T-3 at low concentrations but depressed the luminosity at higher concentrations. Selenite and fulvic acid in combination caused a larger enhancement and a smaller depression of the luminosity than observed with either substance alone. The hypothesis is formulated that Kaschin-Beck disease is caused by selenium levels insufficient to prevent the toxic effects of the organic compounds present in the drinking water of the regions in which the disease is endemic.
为了阐明大骨节病的病因（通过在饮食中补充亚硒酸钠可预防该病），研究了无机硒化合物（亚硒酸盐和硒酸盐）与腐殖酸/富里酸之间的相互作用。发现在酸性溶液中，硒酸盐会被腐殖酸缓慢还原为亚硒酸盐。亚硒酸盐从溶液中吸附到二氧化锰和三氧化二铁上的程度，比吸附到高岭土、腐殖酸、永蜀土壤或二氧化硅上的程度大得多。给小鼠喂食添加了亚硒酸钠的饮食会增加肾脏、肝脏、脾脏、肺、心脏和血液中的硒浓度。含有亚硒酸钠和富里酸的饮食导致除脾脏外各器官中的硒浓度低于仅含亚硒酸钠饮食时观察到的浓度。硒和富里酸会增加谷胱甘肽过氧化物酶的活性。将亚硒酸钠和富里酸联合注入小鼠和大鼠的腹腔，其毒性比单独使用任何一种物质时都低。单独施用亚硒酸盐和富里酸在低浓度时会增强发光菌磷光杆菌T - 3的发光度，但在高浓度时会降低发光度。亚硒酸盐和富里酸联合使用时，与单独使用任何一种物质相比，对发光度的增强作用更大，降低作用更小。由此提出一个假说，即大骨节病是由硒水平不足以预防该病流行地区饮用水中有机化合物的毒性作用所致。
