Huzmeli Can, Bagci Gokhan, Candan Ferhan, Bagci Binnur, Akkaya Lale, Kayatas Mansur
1 Department of Nephrology, Faculty of Medicine, Cumhuriyet University, Sivas, Turkey.
2 Department of Medical Genetics, Faculty of Medicine, Cumhuriyet University, Sivas, Turkey.
J Vasc Access. 2018 May;19(3):303-310. doi: 10.1177/1129729817752860. Epub 2018 Mar 15.
We investigated the influence of the vitamin D receptor gene TaqI (rs731236), ApaI (rs7975232), and FokI (rs2228570) polymorphisms in arteriovenous fistula failure in hemodialysis patients.
This study was carried out with 54 patients who experienced two or more fistula failures in the late period after arteriovenous fistula operation and 58 control patients with no history of arteriovenous fistula failure in 3 years or longer. The polymerase chain reaction-restriction fragment length polymorphism method was used to determine the vitamin D receptor TaqI, FokI, and ApaI polymorphisms.
For vitamin D receptor gene TaqI and Fok1 polymorphisms, no significant association was found between the two groups ( p > 0.05). However, a statistically significant association was determined for ApaI polymorphism between the two groups ( p = 0.02). In patients, ApaI AA, AC, and CC genotype frequencies were found as 21 (38.9%), 32 (59.3%), and 1 (1.8%), respectively. However, genotype frequencies of AA, AC, and CC in the control group were 29 (50%), 22 (37.9%), and 7 (12.1%), respectively. In all three polymorphisms, no significant difference was found between the two groups in terms of allele frequencies ( p > 0.05).
Vitamin D receptor ApaI AC genotype may be a possible cardiovascular risk factor for the development of arteriovenous fistula failure.
我们研究了维生素D受体基因TaqI（rs731236）、ApaI（rs7975232）和FokI（rs2228570）多态性对血液透析患者动静脉内瘘失败的影响。
本研究纳入了54例在动静脉内瘘手术后晚期经历两次或更多次内瘘失败的患者，以及58例在3年或更长时间内无动静脉内瘘失败史的对照患者。采用聚合酶链反应-限制性片段长度多态性方法来确定维生素D受体TaqI、FokI和ApaI多态性。
对于维生素D受体基因TaqI和Fok1多态性，两组之间未发现显著关联（p>0.05）。然而，两组之间ApaI多态性存在统计学显著关联（p = 0.02）。在患者中，ApaI AA、AC和CC基因型频率分别为21（38.9%）、32（59.3%）和1（1.8%）。然而，对照组中AA、AC和CC的基因型频率分别为29（50%）、22（37.9%）和7（12.1%）。在所有三种多态性中，两组之间的等位基因频率无显著差异（p>0.05）。
维生素D受体ApaI AC基因型可能是动静脉内瘘失败发生的一个潜在心血管危险因素。
