尿路上皮型膀胱癌、血浆25-羟基维生素D水平与维生素D受体ApaI、BsmI、FokI和TaqI多态性之间的关系

The Relationship Between Urothelial Type Bladder Cancer, Plasma 25-Hydroxyvitamin D Levels, and Vitamin D Receptor ApaI BsmI FokI, and TaqI Polymorphisms.

作者信息

Baykan Ozgur, Akgul Murat, Uren Nihal, Yaman Ali, Tinay Ilker, Ergul Emel, Sazci Ali, Turkeri Levent, Haklar Goncagül

出版信息

Clin Lab. 2019 Apr 1;65(4). doi: 10.7754/Clin.Lab.2018.180339.

DOI:10.7754/Clin.Lab.2018.180339

PMID:30969078

Abstract

BACKGROUND

Bladder cancer is an important health problem which ranks 4th among most frequently seen cancer types in men. In our study we aimed to investigate the correlations among urothelial type bladder cancer polymorphisms, ApaI, BsmI, FokI, and TaqI, prevalently observed in the vitamin D receptor (VDR) gene and plasma vitamin D levels in a Turkish population.

METHODS

Our study included 101 patients and 109 control subjects. Plasma 25(OH)D levels were determined using a HPLC method and VDR gene polymorphisms with PCR-RFLP method.

RESULTS

A statistically significant intergroup difference was not observed with regard to age, gender, and BMIs of the patients. Median (min - max) 25(OH)D levels in the patient and the control groups were determined as 11.9 ng/dL (1.9 - 33.0 ng/dL) and 9.7 ng/dL (2.1 - 39.5 ng/dL), respectively. A statistically significant intergroup difference was not observed with regard to 25(OH)D levels (p = 0.402). A statistically significant intergroup differ-ence was not observed with regard to genotype distribution of ApaI, BsmI, and TaqI polymorphisms and allele frequencies. Control and urothelial type bladder cancer groups showed a statistically significant difference with respect to genotype distribution of FokI polymorphism (p = 0.048). However in a binary logistic regression model, when corrected OR values were estimated by including smoking history in the model, the correlation detected be-tween the presence of FF and increased risk of disease was not statistically significant (ORadj = 1.64, 95% CI = 0.89 - 3.02, p = 0.114).

CONCLUSIONS

In the light of the data concerning Turkish population a statistically significant correlation could not be demonstrated between plasma vitamin D levels, ApaI, BsmI, FokI, and TaqI polymorphisms, and urothelial type bladder cancers. Since literature data are limited in number, further studies should be conducted in larger patient groups.

摘要

背景

膀胱癌是一个重要的健康问题，在男性最常见的癌症类型中排名第四。在我们的研究中，我们旨在调查在土耳其人群中维生素D受体（VDR）基因中普遍存在的尿路上皮型膀胱癌多态性（ApaI、BsmI、FokI和TaqI）与血浆维生素D水平之间的相关性。

方法

我们的研究纳入了101例患者和109例对照受试者。采用高效液相色谱法测定血浆25（OH）D水平，采用聚合酶链反应-限制性片段长度多态性（PCR-RFLP）方法检测VDR基因多态性。

结果

患者的年龄、性别和体重指数在组间未观察到统计学显著差异。患者组和对照组的25（OH）D水平中位数（最小值 - 最大值）分别确定为11.9 ng/dL（1.9 - 33.0 ng/dL）和9.7 ng/dL（2.1 - 39.5 ng/dL）。25（OH）D水平在组间未观察到统计学显著差异（p = 0.402）。ApaI、BsmI和TaqI多态性的基因型分布及等位基因频率在组间未观察到统计学显著差异。对照组和尿路上皮型膀胱癌组在FokI多态性的基因型分布方面存在统计学显著差异（p = 0.048）。然而，在二元逻辑回归模型中，当通过将吸烟史纳入模型来估计校正后的比值比（OR）值时，检测到的FF存在与疾病风险增加之间的相关性无统计学显著性（ORadj = 1.64，95%可信区间 = 0.89 - 3.02，p = 0.114）。