卵巢中促黄体生成素受体mRNA表达的调控：miR-122的作用。
Regulation of Luteinizing Hormone Receptor mRNA Expression in the Ovary: The Role of miR-122.
作者信息
Menon K M J, Menon Bindu, Gulappa Thippeswamy
机构信息
The University of Michigan Medical School, Ann Arbor, MI, United States.
The University of Michigan Medical School, Ann Arbor, MI, United States.
出版信息
Vitam Horm. 2018;107:67-87. doi: 10.1016/bs.vh.2018.01.010. Epub 2018 Feb 19.
The expression of luteinizing hormone receptor (LHR) in the mammalian ovary is regulated in response to changes in the secretion of follicle-stimulating hormone and luteinizing hormone by the anterior pituitary, at least in part, through posttranscriptional mechanisms. The steady-state levels of LHR mRNA are maintained by controlling its rate of degradation by an RNA-binding protein designated as LHR mRNA-binding protein (LRBP). LRBP forms a complex with LHR mRNA and targets it for degradation in the p bodies. miR-122, an 18 nucleotide noncoding RNA, regulates the expression of LRBP. Thus, the levels of miR-122 determine the cellular levels of LHR mRNA expression. This phenomenon has been examined during the induction of LHR mRNA expression that occurs during follicle maturation in response to rising levels of FSH. In this situation, miR-122 and LRBP levels decrease as LHR mRNA expression undergoes downregulation in response to preovulatory LH surge. miR-122 expression as well as LRBP levels show robust increases. The mechanism of induction of LRBP by miR-122 has also been discussed.
哺乳动物卵巢中促黄体生成素受体（LHR）的表达至少部分通过转录后机制，响应垂体前叶促卵泡激素和促黄体生成素分泌的变化而受到调节。LHR mRNA的稳态水平通过一种名为LHR mRNA结合蛋白（LRBP）的RNA结合蛋白控制其降解速率来维持。LRBP与LHR mRNA形成复合物，并将其靶向到加工小体中进行降解。miR-122是一种18个核苷酸的非编码RNA，可调节LRBP的表达。因此，miR-122的水平决定了LHR mRNA表达的细胞水平。在卵泡成熟过程中，随着FSH水平升高诱导LHR mRNA表达期间，这一现象已得到研究。在这种情况下，随着排卵前LH峰的出现，LHR mRNA表达下调，miR-122和LRBP水平降低。miR-122表达以及LRBP水平则显著升高。还讨论了miR-122诱导LRBP的机制。
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