Mercantepe Tolga, Unal Deniz, Tümkaya Levent, Yazici Zihni Acar
Department of Histology and Embryology, Faculty of Medicine, Recep Tayyip Erdoğan University, Rize 53010, Turkey.
Department of Histology and Embryology, Faculty of Medicine, Atatürk University, Erzurum 25000, Turkey.
Exp Ther Med. 2018 Apr;15(4):3404-3412. doi: 10.3892/etm.2018.5819. Epub 2018 Jan 31.
Cisplatin is an effective antineoplastic drug that is usually used to treat a number of different types of cancer in the clinic. One of the most notable side effects of cisplatin use is infertility. The present study was designed to determine the non-oxidative testicular effects caused by the use of cisplatin in rats. The rats were randomly allocated to the experimental groups. The untreated rats represented the control group (group I) and the treatment groups were as follows: cisplatin alone (group II), cisplatin+amifostine (group III), cisplatin+curcumin (group IV), and cisplatin+caffeic acid phenethyl ester (CAPE; group V). The present study observed that following cisplatin administration, the expression of nuclear factor-κB (NF-κβ)/p65, caspase-3 and 8-deoxyguanosine (8-OHdG) increased in germinal epithelium and Leydig cells. However, the expression of these markers decreased in groups III-V, most notably in the group treated with amifostine. cisplatin induced-damage was countered by amifostine and curcumin. The results revealed that the activation of NF-κB, caspase-3 and 8-OHdG had a significant role in cisplatin-induced testicular toxicity. Thus, amifostine, curcumin and, to a lesser extent, CAPE have the potential for use as therapeutic adjuvants in cisplatin-induced testis injury.
顺铂是一种有效的抗肿瘤药物，临床上通常用于治疗多种不同类型的癌症。使用顺铂最显著的副作用之一是不育。本研究旨在确定大鼠使用顺铂引起的非氧化性睾丸效应。将大鼠随机分配到实验组。未处理的大鼠作为对照组（I组），治疗组如下：单独使用顺铂（II组）、顺铂+氨磷汀（III组）、顺铂+姜黄素（IV组）和顺铂+咖啡酸苯乙酯（CAPE；V组）。本研究观察到，给予顺铂后，生精上皮和睾丸间质细胞中核因子-κB（NF-κβ）/p65、半胱天冬酶-3和8-羟基脱氧鸟苷（8-OHdG）的表达增加。然而，这些标志物的表达在III-V组中降低，最显著的是在氨磷汀治疗组中。氨磷汀和姜黄素可对抗顺铂诱导的损伤。结果表明，NF-κB、半胱天冬酶-3和8-OHdG的激活在顺铂诱导的睾丸毒性中起重要作用。因此，氨磷汀、姜黄素以及程度较轻的CAPE有潜力用作顺铂诱导的睾丸损伤的治疗佐剂。
