Yilmaz Adnan, Dizman Fatih, Akyildiz Kerimali, Mataraci Karakas Sibel, Mercantepe Tolga, Uydu Huseyin Avni, Tumkaya Levent, Ozturk Koksal
Department of Biochemistry, Faculty of Medicine, Recep Tayyip Erdogan University, 53010 Rize, Turkey.
Recep Tayyip Erdogan University Research and Training Hospital, 53010 Rize, Turkey.
Life (Basel). 2024 Aug 28;14(9):1077. doi: 10.3390/life14091077.
Acute liver injury is an increasing global health problem. It is a widespread side effect of cisplatin treatment in the clinic and can lead to liver failure if not treated promptly. Previous studies have revealed that green tea can protect some organs from treatments. However, the potential of white tea to prevent the negative effects of acute liver injury has not been addressed so far. The purpose of this study was to investigate the reduction in cisplatin-induced liver injury in rats receiving white tea. Female Sprague Dawley rats with similar weight were selected in this study. Twenty-four rats were divided into three groups of eight animals each and ad libitum nutrition was provided. The control and cisplatin groups were given tap water only, while the white tea + cisplatin group received white tea at a 0.5% weight/volume concentration for four weeks. At the end of the fourth week, the white tea + cisplatin group and the cisplatin group received a single dose of cisplatin (7 mg/kg) via the intraperitoneal route. Five days after that procedure, the rats were anesthetized. Liver tissues and blood samples were collected, which were used for biochemical and histopathological analyses. According to biochemical results, liver tissue MDA and GSH, serum ALT, and AST levels significantly increased in the cisplatin group compared to the control group. Compared with the cisplatin group, although MDA, AST, ALT, and GSH levels were lower in the white tea + cisplatin group, only GSH levels were statistically different. The examination of histopathological and immunohistochemical findings revealed apoptotic cells, vascular congestion, and sinusoidal dilatation in the cisplatin group compared to the control group. This adverse event decreased in the white tea + cisplatin group compared to the cisplatin group. In conclusion, white tea exhibits an ameliorating effect on cisplatin-induced liver injury.
急性肝损伤是一个日益严重的全球健康问题。它是临床上顺铂治疗的一种普遍副作用，如果不及时治疗可能导致肝衰竭。先前的研究表明绿茶可以保护某些器官免受治疗的影响。然而，白茶预防急性肝损伤负面影响的潜力迄今为止尚未得到研究。本研究的目的是调查接受白茶的大鼠中顺铂诱导的肝损伤的减轻情况。本研究选用了体重相似的雌性Sprague Dawley大鼠。24只大鼠被分为三组，每组8只，并提供自由采食的营养。对照组和顺铂组仅给予自来水，而白茶 + 顺铂组以0.5%重量/体积浓度给予白茶，持续四周。在第四周结束时，白茶 + 顺铂组和顺铂组通过腹腔途径接受单剂量顺铂（7 mg/kg）。在该操作五天后，将大鼠麻醉。收集肝脏组织和血液样本，用于生化和组织病理学分析。根据生化结果，与对照组相比，顺铂组肝脏组织丙二醛（MDA）和谷胱甘肽（GSH）、血清谷丙转氨酶（ALT）和谷草转氨酶（AST）水平显著升高。与顺铂组相比，虽然白茶 + 顺铂组的MDA、AST、ALT和GSH水平较低，但只有GSH水平有统计学差异。组织病理学和免疫组织化学结果检查显示，与对照组相比，顺铂组存在凋亡细胞、血管充血和肝血窦扩张。与顺铂组相比，白茶 + 顺铂组的这种不良事件有所减少。总之，白茶对顺铂诱导的肝损伤具有改善作用。
