Yang Hongxia, Zhang Gang, Che Xiaolin, Yu Shudong
Department of Otorhinolaryngology, Maternal and Child Health Hospital of Tai'an, Tai'an, Shandong 271000, P.R. China.
Department of Otolaryngology, Affiliated Hospital of Taishan Medical University, Tai'an, Shandong 271000, P.R. China.
Exp Ther Med. 2018 Apr;15(4):3477-3482. doi: 10.3892/etm.2018.5844. Epub 2018 Feb 7.
Slug is associated with the radioresistance of nasopharyngeal carcinoma (NPC) and the main current approach of treatment for NPC is radiotherapy. Hence, the aim of the current study was to determine the effect of Slug silencing on the radiosensitivity of NPC cells. Lentiviral-mediated transfection of Slug RNA interference (RNAi) in NPC cell line C666-1 was performed . Following Slug inhibition, its expression was detected using western blotting. A clonogenic survival assay and flow cytometry were then performed to evaluate the clonogenic cell survival, cell cycle distribution and apoptosis of C666-1 cells following irradiation. The results indicated that Slug RNAi decreased cell proliferation, and increased cell apoptosis and G/G arrest. Thus, lentiviral-mediated transfection of Slug RNAi enhanced the radiosensitivity of the NPC cell line C666-1, and Slug may therefore be a potential target to improve radiotherapy in treatment of NPC and reduce the radioresistance of NPC.
Slug与鼻咽癌（NPC）的放射抗性相关，而目前NPC的主要治疗方法是放射治疗。因此，本研究的目的是确定Slug基因沉默对NPC细胞放射敏感性的影响。采用慢病毒介导的方法将Slug RNA干扰（RNAi）转染至NPC细胞系C666-1中。抑制Slug后，通过蛋白质免疫印迹法检测其表达。然后进行克隆形成存活试验和流式细胞术，以评估照射后C666-1细胞的克隆形成细胞存活、细胞周期分布和凋亡情况。结果表明，Slug RNAi降低了细胞增殖，增加了细胞凋亡和G/G期阻滞。因此，慢病毒介导的Slug RNAi转染增强了NPC细胞系C666-1的放射敏感性，因此Slug可能是提高NPC放射治疗效果和降低NPC放射抗性的潜在靶点。
