Wang Chengyu, Wang Xueqiao, Han Angxuan, Wang Yuhao, Jiang Hui
Department of Otolaryngology, Jinshan Hospital, Fudan University, Shanghai 201508, P.R. China.
Research Center for Clinical Medicine, Jinshan Hospital, Fudan University, Shanghai 201508, P.R. China.
Exp Ther Med. 2021 May;21(5):470. doi: 10.3892/etm.2021.9901. Epub 2021 Mar 8.
Nasopharyngeal carcinoma (NPC) is an epithelial carcinoma that arises from the lining of the nasopharyngeal mucosa. The efficacy of radiation therapy is limited due to radiation resistance, particularly in the advanced stages of NPC. The S100P protein is a small isoform of the S100 protein family, which is involved in the regulation of various intracellular and extracellular processes, including proliferation, differentiation and intracellular signaling. The aim of the current study was to investigate the significance of the S100P-RAGE axis in NPC progression. The expression levels of S100P and receptor for activated glycation end-products (RAGE) in NPC specimens were determined by western blotting. In addition, the effect of the S100P-RAGE axis on NPC was evaluated by proliferation and migration assays using C666-1 cells treated with S100P or the RAGE inhibitor FPS-ZM1. The underlying mechanism was also investigated by western blotting. The expression of S100P and RAGE was detected in clinical specimens from 15 patients with NPC and 15 patients with benign nasopharyngeal inflammation, and was observed to be higher in NPC tissues compared with inflamed tissues. Furthermore, the interaction of S100P with RAGE increased the proliferation and migration potential of C666-1 cells, and activated mitogen-activated protein kinase and NF-κB signaling. These results indicate that the S100P-RAGE axis exerts a promoting effect on the progression of NPC. Therefore therapeutic strategies targeting S100P-RAGE merit further exploration for the treatment of NPC.
鼻咽癌（NPC）是一种起源于鼻咽黏膜上皮的上皮性癌。由于放射抗性，放射治疗的疗效有限，尤其是在鼻咽癌的晚期阶段。S100P蛋白是S100蛋白家族的一种小异构体，参与多种细胞内和细胞外过程的调节，包括增殖、分化和细胞内信号传导。本研究的目的是探讨S100P-RAGE轴在鼻咽癌进展中的意义。通过蛋白质免疫印迹法测定鼻咽癌标本中S100P和晚期糖基化终产物受体（RAGE）的表达水平。此外，使用经S100P或RAGE抑制剂FPS-ZM1处理的C666-1细胞，通过增殖和迁移试验评估S100P-RAGE轴对鼻咽癌的影响。还通过蛋白质免疫印迹法研究了潜在机制。在15例鼻咽癌患者和15例鼻咽良性炎症患者的临床标本中检测到S100P和RAGE的表达，与炎症组织相比，在鼻咽癌组织中观察到其表达更高。此外，S100P与RAGE的相互作用增加了C666-1细胞的增殖和迁移潜能，并激活了丝裂原活化蛋白激酶和NF-κB信号传导。这些结果表明，S100P-RAGE轴对鼻咽癌的进展具有促进作用。因此，针对S100P-RAGE的治疗策略值得进一步探索用于鼻咽癌的治疗。
