Tsui Y P, Shea Graham K, Chan Y S, Shum Daisy K Y
School of Biomedical Sciences, LKS Faculty of Medicine, The University of Hong Kong, Hong Kong, China.
Department of Orthopaedics and Traumatology, LKS Faculty of Medicine, The University of Hong Kong, Hong Kong, China.
Methods Mol Biol. 2018;1739:137-148. doi: 10.1007/978-1-4939-7649-2_9.
Our goal is to derive phenotypically stable Schwann cells from bone marrow stromal cells (BMSCs) for use in transplantation studies of central/peripheral nerve injuries. With the adult rat as model, here we describe steps that foster (1) expansion of the BMSC subpopulation of neural progenitors as neurosphere cells, (2) differentiation of the progenitors into Schwann cell-like cells in adherent culture supplemented with soluble factors, and (3) cell-intrinsic switch of Schwann cell-like cells to the Schwann cell fate following co-culture with sensory neurons purified from dorsal root ganglia. The derived Schwann cells retain marker expression despite withdrawal of supplements and neuronal cues, survive passaging and cryopreservation, and, importantly, show functional capacity for myelination.
我们的目标是从骨髓基质细胞（BMSCs）中获得表型稳定的施万细胞，用于中枢/外周神经损伤的移植研究。以成年大鼠为模型，在此我们描述了促进以下过程的步骤：（1）将神经祖细胞的BMSC亚群作为神经球细胞进行扩增；（2）在添加可溶性因子的贴壁培养中，将祖细胞分化为施万细胞样细胞；（3）与从背根神经节纯化的感觉神经元共培养后，施万细胞样细胞发生细胞内源性转变，转变为施万细胞命运。尽管去除了补充剂和神经元信号，所获得的施万细胞仍保留标记物表达，能够在传代和冷冻保存后存活，并且重要的是，显示出髓鞘形成的功能能力。
