Cai Sa, Shum Daisy K Y, Chan Ying-Shing
School of Biomedical Sciences, Li Ka Shing Faculty of Medicine, The University of Hong Kong, Hong Kong, China.
Methods Mol Biol. 2018;1739:149-160. doi: 10.1007/978-1-4939-7649-2_10.
Here we describe the in vitro derivation of sensory neurons for use in effecting fate commitment of Schwann cell-like cells derived from human bone marrow stromal cells (hBMSCs). We adopt a novel combination of small molecules in an 8-day program that induces the differentiation of human induced pluripotent stem cells into sensory neurons. In co-cultures, the derived sensory neurons present contact-dependent cues to direct hBMSC-derived Schwann cell-like cells toward the Schwann cell fate. These derived human Schwann cells survive passaging and cryopreservation, retain marker expression despite withdrawal of glia-inducing medium and neuronal cues, demonstrate capacity for myelination, and therefore promise application in autologous transplantation and re-myelination therapy.
在此，我们描述了用于诱导源自人骨髓基质细胞（hBMSC）的雪旺氏细胞样细胞发生命运转变的感觉神经元的体外衍生方法。我们在一个为期8天的方案中采用了小分子的新型组合，该方案可诱导人诱导多能干细胞分化为感觉神经元。在共培养中，衍生的感觉神经元呈现接触依赖性信号，以引导源自hBMSC的雪旺氏细胞样细胞走向雪旺氏细胞命运。这些衍生的人雪旺氏细胞在传代和冷冻保存后仍能存活，尽管去除了胶质细胞诱导培养基和神经元信号，仍保留标志物表达，表现出髓鞘形成能力，因此有望应用于自体移植和再髓鞘化治疗。
