School of Biomedical Sciences, Li Ka Shing Faculty of Medicine, The University of Hong Kong, Hong Kong, Hong Kong SAR.
Department of Orthopaedics & Traumatology, Li Ka Shing Faculty of Medicine, The University of Hong Kong, Hong Kong, Hong Kong SAR.
Stem Cell Reports. 2017 Oct 10;9(4):1097-1108. doi: 10.1016/j.stemcr.2017.08.004. Epub 2017 Sep 7.
Our ultimate goal of in vitro derivation of Schwann cells (SCs) from adult bone marrow stromal cells (BMSCs) is such that they may be used autologously to assist post-traumatic nerve regeneration. Existing protocols for derivation of SC-like cells from BMSCs fall short in the stability of the acquired phenotype and the functional capacity to myelinate axons. Our experiments indicated that neuro-ectodermal progenitor cells among the human hBMSCs could be selectively expanded and then induced to differentiate into SC-like cells. Co-culture of the SC-like cells with embryonic dorsal root ganglion neurons facilitated contact-mediated signaling that accomplished the switch to fate-committed SCs. Microarray analysis and in vitro myelination provided evidence that the human BMSC-derived SCs were functionally mature. This was reinforced by repair and myelination phenotypes observable in vivo with the derived SCs seeded into a nerve guide as an implant across a critical gap in a rat model of sciatic nerve injury.
我们的最终目标是从成人骨髓基质细胞（BMSCs）体外诱导雪旺细胞（SCs），以便可以将其自体用于辅助创伤后神经再生。现有的从 BMSCs 诱导 SC 样细胞的方案在获得的表型稳定性和髓鞘化轴突的功能能力方面存在不足。我们的实验表明，人 hBMSCs 中的神经外胚层祖细胞可以被选择性地扩增，然后被诱导分化为 SC 样细胞。将 SC 样细胞与胚胎背根神经节神经元共培养，促进了接触介导的信号转导，从而实现了向命运决定的 SC 的转变。微阵列分析和体外髓鞘形成提供的证据表明，人 BMSC 来源的 SC 具有功能成熟性。这一点通过在大鼠坐骨神经损伤模型中，将衍生的 SC 种植到神经导管中作为植入物跨越关键间隙，在体内观察到的修复和髓鞘化表型得到了加强。
