Sağıroğlu Pınar, Hasdemir Ufuk, Altınkanat Gelmez Gülşen, Aksu Burak, Karatuna Onur, Söyletir Güner
Marmara University School of Medicine, Department of Medical Microbiology, Istanbul, Turkey; Private Melikgazi Hospital, Medical Microbiology, Kayseri, Turkey.
Marmara University School of Medicine, Department of Medical Microbiology, Istanbul, Turkey.
Braz J Microbiol. 2018 Oct-Dec;49(4):885-890. doi: 10.1016/j.bjm.2018.02.002. Epub 2018 Mar 1.
In this study, the performance of the "RESIST-3 O.K.N. K-SeT" (Coris BioConcept, Gembloux, Belgium) immunochromatographic assay was evaluated in 132 Klebsiella pneumoniae comprising 102 carbapenem resistant and 30 carbapenem susceptible isolates. Genotypically known isolates of Gram negative bacteria (n=22) including various species were also tested by the assay as controls. The isolates tested by the immunochromatographic assay and also were run PCR for bla, bla, bla, bla, and bla. The rates of bla, bla, and bla in carbapenem resistant isolates were found at 52.9%, 39.2%, and 2.0%, respectively. Both bla and bla were found in six (5.9%) isolates. The results of the assay showed 100% concordance with those obtained by PCR in 132K. pneumoniae. The agreement between the two methods was found to be identical at the isolate level. The assay also correctly detected all genotypically known isolates of Escherichia coli, Serratia marcescens, Citrobacter freundii, Enterobacter cloacae, K. pneumoniae carrying bla, bla, and/or bla. On the other hand, the assay did not exhibit any cross-reaction in control isolates harboring bla and bla. We conclude that the RESIST-3 O.K.N. K-SeT is a reliable, rapid, and user friendly test and we recommend it for routine diagnostic laboratories.
在本研究中，对“RESIST-3 O.K.N. K-SeT”（比利时热姆布卢的科里斯生物概念公司）免疫层析检测法在132株肺炎克雷伯菌中的性能进行了评估，其中包括102株耐碳青霉烯类菌株和30株对碳青霉烯类敏感的菌株。还对包括各种菌种在内的22株基因型已知的革兰氏阴性菌分离株进行了该检测法检测作为对照。对经免疫层析检测法检测的分离株也进行了bla、bla、bla、bla和bla的PCR检测。耐碳青霉烯类分离株中bla、bla和bla的检出率分别为52.9%、39.2%和2.0%。在6株（5.9%）分离株中同时检测到bla和bla。检测结果显示，在132株肺炎克雷伯菌中，该检测法与PCR检测结果的一致性为100%。两种方法在分离株水平上的一致性相同。该检测法还正确检测出了所有基因型已知的携带bla、bla和/或bla的大肠杆菌、粘质沙雷氏菌、弗氏柠檬酸杆菌、阴沟肠杆菌、肺炎克雷伯菌分离株。另一方面，该检测法在携带bla和bla的对照分离株中未表现出任何交叉反应。我们得出结论，RESIST-3 O.K.N. K-SeT是一种可靠、快速且用户友好的检测方法，我们推荐将其用于常规诊断实验室。
