不明原因复发性自然流产女性与正常生育女性血清中Th17和Treg细胞因子水平的比较
Comparing Serum Levels of Th17 and Treg Cytokines in Women with Unexplained Recurrent Spontaneous Abortion and Fertile Women.
作者信息
Roomandeh Narges, Saremi AboTaleb, Arasteh Javad, Pak Fatemeh, Mirmohammadkhani Majid, Kokhaei Parviz, Zare Ahad
机构信息
Student Research Committee and Department of Immunology, Faculty of Medicine, Semnan University of Medical Sciences, Semnan, Iran.
出版信息
Iran J Immunol. 2018 Mar;15(1):59-67.
BACKGROUND
Increased evidences have shown that unexplained recurrent spontaneous abortion (URSA) is associated with inflammatory responses and breakage of immunological autotolerance. Therefore, the balance between Th17 and Treg cells may elucidate the pathophysiology of URSA.
OBJECTIVE
To investigate the serum concentration of regulatory and inflammatory cytokines associated with Treg and Th17 in both normal and URSA females.
METHODS
Forty-six women with URSA and 28 non-pregnant control women with at least one successful pregnancy were included. Serum was obtained from both groups and stored at -7º C. The serum concentrations of IL-17, IL-21, IL-22, IL-10, and TGF-β were quantitatively determined by ELISA.
RESULTS
The levels of IL-17, IL-21, and IL-22 in sera were significantly higher (P<0.001, P=0.01 and P<0.001, respectively) and TGF-β serum concentration was significantly lower (P=0.02) in URSA women compared with normal controls.
CONCLUSION
Our results suggest that enhancement in Th17-associated cytokine levels and reduction in TGF-β may be one of the factors involved in URSA.
背景
越来越多的证据表明，原因不明的复发性自然流产（URSA）与炎症反应和免疫自身耐受的破坏有关。因此，Th17细胞与调节性T细胞（Treg）之间的平衡可能有助于阐明URSA的病理生理学机制。
目的
研究正常女性和URSA女性中与Treg及Th17相关的调节性细胞因子和炎性细胞因子的血清浓度。
方法
纳入46例URSA女性患者和28例至少有一次成功妊娠的未孕对照女性。采集两组的血清并储存于-7℃。采用酶联免疫吸附测定法（ELISA）定量测定血清中白细胞介素17（IL-17）、白细胞介素21（IL-21）、白细胞介素22（IL-22）、白细胞介素10（IL-10）和转化生长因子β（TGF-β）的浓度。
结果
与正常对照相比，URSA女性血清中IL-17、IL-21和IL-22水平显著升高（分别为P<0.001、P=0.01和P<0.001），而TGF-β血清浓度显著降低（P=0.02）。
结论
我们的研究结果表明，Th17相关细胞因子水平升高和TGF-β降低可能是URSA的影响因素之一。
Zotero 插件