Fontana L, Fattorossi A, D'Amelio R, Migliorati A, Perricone R
Immunopharmacology. 1987 Apr;13(2):111-5. doi: 10.1016/0162-3109(87)90047-6.
The effects of physiological concentrations of beta-endorphin on the proliferative response to concanavalin A of human peripheral blood mononuclear cells from a large series of healthy donors are reported. These effects are also compared with those obtained employing beta-endorphin and phytohemagglutinin under the same experimental conditions. The donors (32), aged between 20 and 48 years, chosen among military personnel of the Italian Air Force, underwent clinical and laboratory investigations to exclude any detectable disturbance in their psychophysical fitness. Our results show that beta-endorphin is not mitogenic per se and is unable to modify the response of mononuclear cells to phytohemagglutinin irrespective of the concentration of opioid or mitogen used. beta-Endorphin is also unable to alter the PBMC response to low concentrations of concanavalin A, but significantly increases such a response when higher concentrations of concanavalin A and concentrations of beta-endorphin similar to those found in human plasma under physiological conditions are used. The effect is not reverted by naloxone, the specific opiate antagonist. When the activity of beta-endorphin on the mononuclear cell response to concanavalin A is examined at the single donor level, it is noted that some of the donors fail to show the opioid-dependent increase. The baseline levels of the response to concanavalin A of such subjects, compared to those of the donors whose response is augmented by the opioid, are significantly higher, thus demonstrating that beta-endorphin can selectively modulate concanavalin A-induced mitogenesis with a behavior depending on the individual characteristics of the donor's response. The process involves non-opioid cell receptors.
报告了生理浓度的β-内啡肽对来自大量健康供体的人外周血单个核细胞对伴刀豆球蛋白A增殖反应的影响。还将这些影响与在相同实验条件下使用β-内啡肽和植物血凝素所获得的影响进行了比较。供体（32名）年龄在20至48岁之间，从意大利空军军事人员中挑选，接受了临床和实验室检查，以排除其心理生理健康方面的任何可检测到的干扰。我们的结果表明，β-内啡肽本身不具有促有丝分裂作用，并且无论使用的阿片类药物或促有丝分裂原浓度如何，都无法改变单个核细胞对植物血凝素的反应。β-内啡肽也无法改变外周血单个核细胞对低浓度伴刀豆球蛋白A的反应，但当使用更高浓度的伴刀豆球蛋白A和与生理条件下人体血浆中发现的浓度相似的β-内啡肽浓度时，会显著增加这种反应。该作用不能被特异性阿片拮抗剂纳洛酮逆转。当在单个供体水平上检查β-内啡肽对单个核细胞对伴刀豆球蛋白A反应的活性时，注意到一些供体未显示出阿片类药物依赖性增加。与那些反应因阿片类药物而增强的供体相比，这些受试者对伴刀豆球蛋白A反应的基线水平显著更高，从而表明β-内啡肽可以根据供体反应的个体特征，通过一种行为选择性地调节伴刀豆球蛋白A诱导的有丝分裂。该过程涉及非阿片类细胞受体。
