Department of Biology, Millersville University, Millersville, PA, USA.
Department of Entomology, Michigan State University, East Lansing, MI, USA.
Microb Ecol. 2018 Oct;76(3):719-728. doi: 10.1007/s00248-018-1175-3. Epub 2018 Mar 17.
Ephemeral aquatic habitats and their associated microbial communities (microbiomes) play important roles in the growth and development of numerous aquatic insects, including mosquitoes (Diptera). Biological control agents, such as Bacillus thuringiensis israelensis (Bti) or insect growth regulators (e.g., methoprene), are commonly used to control mosquitoes in these habitats. However, it is unknown how commonly used control compounds affect the mosquito internal microbiome and potentially alter their life history traits. The objectives of this study were threefold: characterize the internal microbiota of Aedes larvae (Culicidae) in ephemeral forested mosquito habitat using high-throughput amplicon based sequencing, assess how mosquito control treatments affect the internal microbial communities of larval mosquitoes, and determine if changes to the microbiome resulted from direct or indirect treatment effects. The larval microbiome varied in community composition and diversity with development stage and treatment, suggesting potential effects of control compounds on insect microbial ecology. While microbial community differences due to Bti treatment were a result of indirect effects on larval development, methoprene had significant impacts on bacterial and algal taxa that could not be explained by indirect treatment effects. These results provide new information on the interactions between pesticide treatments and insect microbial communities.
短暂性水生栖息地及其相关的微生物群落（微生物组）在许多水生昆虫的生长和发育中起着重要作用，包括蚊子（双翅目）。生物防治剂，如苏云金芽孢杆菌以色列亚种（Bti）或昆虫生长调节剂（如灭幼脲），通常用于控制这些栖息地中的蚊子。然而，目前尚不清楚常用的控制化合物如何影响蚊子的内部微生物组，并可能改变它们的生活史特征。本研究的目的有三个：使用高通量扩增子测序技术描述短暂性森林蚊栖息地中伊蚊幼虫（蚊科）的内部微生物群，评估蚊子控制处理如何影响幼虫蚊子的内部微生物群落，并确定微生物组的变化是否来自直接或间接的处理效果。幼虫微生物组的群落组成和多样性随发育阶段和处理而变化，这表明控制化合物可能对昆虫微生物生态学产生影响。虽然 Bti 处理引起的微生物群落差异是幼虫发育间接作用的结果，但灭幼脲对细菌和藻类分类群有显著影响，这些影响不能用间接处理效应来解释。这些结果提供了有关农药处理与昆虫微生物群落之间相互作用的新信息。
