Department of Neurobiology and Acupuncture Research, The Third Clinical Medical College, Zhejiang Chinese Medical University, Hangzhou 310053, China.
Department of Neurobiology and Acupuncture Research, The Third Clinical Medical College, Zhejiang Chinese Medical University, Hangzhou 310053, China.
Life Sci. 2018 May 1;200:69-80. doi: 10.1016/j.lfs.2018.03.028. Epub 2018 Mar 14.
Observing the parameter-specific anti-hyperalgesic effects of EA with different stimulation times and frequencies on painful hyperalgesia mediated by the level of TRPV1 and P2X3 expression in DRG after CFA injection.
The model was induced by the injection of CFA in each rat's right hind paw. EA treatment was applied to the bilateral ST36 and BL60. Paw withdrawal threshold (PWT) and paw withdrawal latency (PWL) were tested with Von Frey filaments and the radiant heat source of the test instrument, respectively. TRPV1 and P2X3 expressions were measured by immunofluorescence and western blot. αβ-meATP and capsaicine combined with EA were further utilized to investigate the change in PWL.
Different stimulation times (20, 30, 45 min) combined with different frequencies (2 Hz, 100 Hz, 2/100 Hz) of EA have analgesic effects on the PWT and PWL; however, the level of the hypoalgesic efficacy of EA was primarily associated with EA frequency. The analgesic effect of EA was better at 100 Hz than at 2 Hz. The level of regulation of 100 Hz EA on TRPV1 and P2X3 in DRG was greater than that of 2 Hz. Furthermore, both TRPV1 agonist and P2X3 agonist may impair the level of EA analgesia.
EA has a parameter-specific effect on chronic inflammatory pain relief, which primarily depend on the stimulation frequency and not on the stimulation time at a certain stimulation time. The parameter-specific analgesic effect of EA is at least partially related to mediation of the protein level of TRPV1 and P2X3 expression in DRG of CFA rats.
观察不同刺激时间和频率的电针（EA）对 CFA 注射后 DRG 中 TRPV1 和 P2X3 表达水平介导的痛觉过敏的参数特异性抗痛觉过敏作用。
通过在每只大鼠右后爪注射 CFA 来诱导模型。在双侧 ST36 和 BL60 上进行 EA 治疗。使用 Von Frey 细丝和测试仪器的辐射热源分别测试爪回缩阈值（PWT）和爪回缩潜伏期（PWL）。通过免疫荧光和 Western blot 测量 TRPV1 和 P2X3 的表达。进一步使用 αβ-meATP 和辣椒素联合 EA 来研究 PWL 的变化。
不同的刺激时间（20、30、45 分钟）与不同的频率（2 Hz、100 Hz、2/100 Hz）的 EA 对 PWT 和 PWL 均具有镇痛作用；然而，EA 低敏效果的水平主要与 EA 频率有关。100 Hz 的 EA 镇痛效果优于 2 Hz。EA 对 DRG 中 TRPV1 和 P2X3 的调节水平在 100 Hz 时高于 2 Hz。此外，TRPV1 激动剂和 P2X3 激动剂都可能损害 EA 镇痛水平。
EA 对慢性炎症性疼痛缓解具有参数特异性效应，这主要取决于刺激频率，而与特定刺激时间的刺激时间无关。EA 的参数特异性镇痛作用至少部分与 CFA 大鼠 DRG 中 TRPV1 和 P2X3 表达的蛋白水平介导有关。
