人血清中的可溶性循环Fcγ受体。一种用于特异性定量检测的新型酶联免疫吸附测定法。
Soluble circulating Fc gamma receptors in human serum. A new ELISA assay for specific and quantitative detection.
作者信息
Khayat D, Geffrier C, Yoon S, Scigliano E, Soubrane C, Weil M, Unkeless J C, Jacquillat C
出版信息
J Immunol Methods. 1987 Jun 26;100(1-2):235-41. doi: 10.1016/0022-1759(87)90194-3.
We have developed a simple, rapid, and sensitive enzyme-linked immunoadsorbent assay (ELISA) to measure soluble cell-free human Fc gamma receptor (Fc gamma R) in serum. This assay is based on the use of two monoclonal antibodies directed against different epitopes expressed on the same low avidity human Fc gamma R (CD16), which is present on polymorphonuclear leukocytes, macrophages and NK cells. This sandwich ELISA, which can measure 2 nM concentration of Fc gamma R, has enabled us to demonstrate the presence and to measure the level of soluble cell-free human Fc gamma R (CfH-Fc gamma R) in normal human serum.
我们开发了一种简单、快速且灵敏的酶联免疫吸附测定法（ELISA），用于测量血清中可溶性无细胞人Fcγ受体（FcγR）。该测定法基于使用两种单克隆抗体，它们针对表达于同一低亲和力人FcγR（CD16）上的不同表位，这种受体存在于多形核白细胞、巨噬细胞和NK细胞上。这种夹心ELISA能够测量2 nM浓度的FcγR，使我们能够证明正常人血清中可溶性无细胞人FcγR（CfH-FcγR）的存在并测量其水平。
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