Peltz G A, Grundy H O, Lebo R V, Yssel H, Barsh G S, Moore K W
Department of Immunology, DNAX Research Institute of Molecular and Cellular Biology, Palo Alto, CA 94304.
Proc Natl Acad Sci U S A. 1989 Feb;86(3):1013-7. doi: 10.1073/pnas.86.3.1013.
A cDNA clone encoding a human receptor for the Fc portion of IgG (Fc gamma R), Fc gamma RIII or CD16, was isolated from a human leukocyte library by a transient expression-immunoselection procedure. This cDNA (pGP5) encodes a 46-kDa phosphatidylinositol-linked cell surface protein with CD16 determinants and affinity for human IgG. The deduced protein sequence is most homologous to the murine receptor Fc gamma RII alpha, with slightly less homology to the human receptors Fc gamma RII and Fc epsilon RI. The cDNA hybridizes to a 2.2 kilobase mRNA in human leukocytes and a cloned human natural killer cell line. Fc gamma RIII is mapped to chromosome 1 by spot-blot analysis of sorted human chromosomes. Hybridization of Fc gamma RII and Fc gamma RIII probes to restriction digests of human genomic DNA separated by pulsed-field gel electrophoresis demonstrates physical linkage of the two genes within a maximum distance of 200 kilobases. The results identify a locus for at least two Fc gamma R genes on human chromosome 1.
通过瞬时表达-免疫选择程序，从人白细胞文库中分离出一个编码人IgG（FcγR）Fc段受体、FcγRIII或CD16的cDNA克隆。该cDNA（pGP5）编码一种具有CD16决定簇且与人IgG有亲和力的46 kDa磷脂酰肌醇连接的细胞表面蛋白。推导的蛋白质序列与小鼠受体FcγRIIα最同源，与人受体FcγRII和FcεRI的同源性略低。该cDNA与人白细胞和克隆的人自然杀伤细胞系中的2.2千碱基mRNA杂交。通过对分选的人染色体进行斑点印迹分析，将FcγRIII定位于1号染色体。用脉冲场凝胶电泳分离的人基因组DNA的限制性酶切片段与FcγRII和FcγRIII探针杂交，证明这两个基因在最大200千碱基的距离内存在物理连锁。结果确定了人1号染色体上至少两个FcγR基因的一个位点。
