Leng Dong, Miao Ran, Huang Xiaoxi, Wang Ying
Clinical Laboratory, Beijing Chao-Yang Hospital, Capital Medical University, Beijing 100020, P.R. China.
Medical Research Center, Beijing Chao-Yang Hospital, Capital Medical University, Beijing 100020, P.R. China.
Oncol Lett. 2018 Apr;15(4):5167-5174. doi: 10.3892/ol.2018.7969. Epub 2018 Feb 6.
Octamer-binding protein 2 (Oct2) binds to the ATGCAAAT octamer on the IgH enhancer and stimulates IgH expression in human multiple myeloma (MM). Cysteine-rich secreted protein 3 (CRISP3) possesses the ATGCAAAT sequence and thus is activated by Oct2 in mouse B cells, suggesting that CRISP3 may be activated in and be a potential biomarker for MM. The present study involved a meta-analysis of the gene expression profiling data of human MM peripheral blood. Significantly expressed genes were analyzed on merged super array microarray data and selected sample data with significantly expressed genes were additionally analyzed by principal component analysis and Bayesian probit regression. CRISP3, Oct2, Apha-1B-glycoprotein (A1GB) and Cyclin D2 (CCND2) were validated in clinical MM peripheral blood samples using reverse transcription quantitative polymerase chain reaction. In the gene expression profiling data, CRISP3 was significantly upregulated and had certain proportions on the discriminated principal component of significantly expressed gene sample data. RT-qPCR analysis revealed CRISP3 was significantly upregulated in MM. Therefore, CRISP3 is a potential peripheral blood biomarker for MM.
八聚体结合蛋白2（Oct2）与免疫球蛋白重链（IgH）增强子上的ATGCAAAT八聚体结合，并刺激人多发性骨髓瘤（MM）中的IgH表达。富含半胱氨酸的分泌蛋白3（CRISP3）拥有ATGCAAAT序列，因此在小鼠B细胞中被Oct2激活，这表明CRISP3可能在MM中被激活并成为MM的潜在生物标志物。本研究对人MM外周血的基因表达谱数据进行了荟萃分析。对合并的超级阵列微阵列数据上显著表达的基因进行分析，并对具有显著表达基因的选定样本数据通过主成分分析和贝叶斯概率回归进行额外分析。使用逆转录定量聚合酶链反应在临床MM外周血样本中验证了CRISP3、Oct2、α-1B-糖蛋白（A1GB）和细胞周期蛋白D2（CCND2）。在基因表达谱数据中，CRISP3显著上调，并且在显著表达基因样本数据的判别主成分上占有一定比例。逆转录定量聚合酶链反应分析显示CRISP3在MM中显著上调。因此，CRISP3是MM的一种潜在外周血生物标志物。
