Han Yuefeng, Zhang Mingjie, Chen Deshang, Li Hui, Wang Xiaomin, Ma Shiyin
Department of Otorhinolaryngology Head and Neck Surgery, The First Affiliated Hospital of Bengbu Medical College, Bengbu, Anhui 233000, P.R. China.
Oncol Lett. 2018 Apr;15(4):5680-5684. doi: 10.3892/ol.2018.8012. Epub 2018 Feb 9.
RNA binding motif protein 17 (RBM17) is a protein-coding gene. The protein encoded by RBM17 is involved in the regulation of alternative splicing and is overexpressed in cancer. The present study aimed to determine the effect of RBM17-knockdown in hypopharyngeal carcinoma FaDu cells using the lentivirus-mediated shRNA method. Cell proliferation was detected by an MTT assay. Flow cytometry analysis was used to determine cell cycle distribution and apoptosis. The results of the present study demonstrated that RBM17 expression was significantly decreased in FaDu cells infected with lentivirus-shRNA. Knockdown of RBM17 expression by shRNA significantly reduced cell proliferation, augmented cell apoptosis and arrested cells at the G/M phase in FaDu cells. The results of the present study indicate that RBM17 serves a notable role in cell proliferation, cell cycle progression and apoptosis of hypopharyngeal carcinoma cells.
RNA结合基序蛋白17（RBM17）是一个蛋白质编码基因。RBM17编码的蛋白质参与可变剪接的调控，且在癌症中过表达。本研究旨在采用慢病毒介导的短发夹RNA（shRNA）方法，确定敲低RBM17对下咽癌FaDu细胞的影响。通过MTT法检测细胞增殖。采用流式细胞术分析确定细胞周期分布和细胞凋亡情况。本研究结果表明，感染慢病毒-shRNA的FaDu细胞中RBM17表达显著降低。shRNA敲低RBM17表达可显著降低FaDu细胞的增殖，增加细胞凋亡，并使细胞停滞于G/M期。本研究结果表明，RBM17在下咽癌细胞的细胞增殖、细胞周期进程和细胞凋亡中发挥显著作用。
