基于飞秒激光的成像引导显微手术在生物组织中产生了新的荧光化合物。
imaging-guided microsurgery based on femtosecond laser produced new fluorescent compounds in biological tissues.
作者信息
Sun Qiqi, Qin Zhongya, Wu Wanjie, Lin Yue, Chen Congping, He Sicong, Li Xuesong, Wu Zhenguo, Luo Yi, Qu Jianan Y
机构信息
Department of Electronic and Computer Engineering, Hong Kong University of Science and Technology, Clear Water Bay, Kowloon, Hong Kong SAR, China.
These authors contributed equally to this work.
出版信息
Biomed Opt Express. 2018 Jan 11;9(2):581-590. doi: 10.1364/BOE.9.000581. eCollection 2018 Feb 1.
Femtosecond laser microsurgery has become an advanced method for clinical procedures and biological research. The tissue treated by femtosecond laser can become highly fluorescent, indicating the formation of new fluorescent compounds that can naturally label the treated tissue site. We systematically characterized the fluorescence signals produced by femtosecond laser ablation in biological tissues . Our findings showed that they possess unique fluorescence properties and can be clearly differentiated from endogenous signals and major fluorescent proteins. We further demonstrated that the new fluorescent compounds can be used as labelling agent for biological imaging and guided laser microsurgery.
飞秒激光显微手术已成为临床手术和生物学研究的一种先进方法。经飞秒激光处理的组织会变得具有高度荧光性，这表明形成了能够自然标记处理过的组织部位的新荧光化合物。我们系统地表征了生物组织中飞秒激光烧蚀产生的荧光信号。我们的研究结果表明，它们具有独特的荧光特性，并且可以与内源性信号和主要荧光蛋白明显区分开来。我们进一步证明，这种新的荧光化合物可以用作生物成像和引导激光显微手术的标记剂。
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