Wang Bao-Gui, Riemann Iris, Schubert Harald, Schweitzer Dietrich, König Karsten, Halbhuber Karl-Juergen
Lasermicroscopy Research Unit, Institute of Microscopic Anatomy (Anatomy II), Friedrich-Schiller University, Teichgraben 7, 07743 Jena, Germany.
Lasers Surg Med. 2007 Jul;39(6):527-33. doi: 10.1002/lsm.20523.
Multiphoton microscopy/tomography has been used as a novel diagnostic method for corneal imaging with subcellular resolution. Here, we used this technique to monitor femtosecond laser intratissue surgery effects.
Multiphoton microscopy/tomography on rabbits based on intense 90 MHz femtosecond Ti: sapphire laser was realized at intensities of MW-GW/cm(2), whereas the surgical procedures were performed with the same system at a higher light intensity on the order of TW/cm(2).
Multiphoton microscopy/tomography proved capable of determining the target of interest, and of visualizing and optically evaluating the in vivo intrastromal laser surgical outcomes with high efficiency. More interesting, using this technique, activated intrastromal keratocytes (myofibroblasts) were detected in vivo 24 hours after intrastromal surgery.
Multiphoton microscopy/tomography is an efficient and convenient non-invasive imaging method which can be used not only to assess intrastromal microsurgical performance but also to perform in vivo follow-up observations on wound repair.
多光子显微镜/断层扫描已被用作一种具有亚细胞分辨率的角膜成像新诊断方法。在此，我们使用该技术来监测飞秒激光组织内手术的效果。
基于强90MHz飞秒钛宝石激光对兔子进行多光子显微镜/断层扫描，其强度为MW - GW/cm²，而手术操作则使用同一系统在更高的光强度（TW/cm²量级）下进行。
多光子显微镜/断层扫描证明能够确定感兴趣的目标，并高效地可视化和光学评估体内基质内激光手术的结果。更有趣的是，使用该技术，在基质内手术后24小时在体内检测到活化的基质角膜细胞（肌成纤维细胞）。
多光子显微镜/断层扫描是一种高效且便捷的非侵入性成像方法，不仅可用于评估基质内显微手术操作，还可对伤口修复进行体内随访观察。
