飞秒激光手术在生物组织中产生的新型荧光化合物：作用机制
New fluorescent compounds produced by femtosecond laser surgery in biological tissues: the mechanisms.
作者信息
Qin Zhongya, Sun Qiqi, Lin Yue, He Sicong, Li Xuesong, Chen Congping, Wu Wanjie, Luo Yi, Qu Jianan Y
机构信息
Department of Electronic and Computer Engineering, Hong Kong University of Science and Technology, Clear Water Bay, Kowloon, Hong Kong SAR, China.
These authors contributed equally to this work.
出版信息
Biomed Opt Express. 2018 Jun 26;9(7):3373-3390. doi: 10.1364/BOE.9.003373. eCollection 2018 Jul 1.
The femtosecond laser ablation in biological tissue produces highly fluorescent compounds that are of great significance for intrinsically labelling ablated tissue and achieving imaging-guided laser microsurgery. In this study, we analyzed the molecular structures of femtosecond laser-ablated tissues using Raman spectroscopy and transmission electron microscopy. The results showed that though laser ablation caused carbonization, no highly fluorescent nanostructures were found in the ablated tissues. Further, we found that the fluorescence properties of the newly formed compounds were spatially heterogeneous across the ablation site and the dominant fluorescent signals exhibited close similarity to the tissue directly heated at a temperature of 200 °C. The findings of our study indicated that the new fluorescent compounds were produced via the laser heating effect and their formation mechanism likely originated from the Maillard reaction, a chemical reaction between amino acids and reducing sugars in tissue.
飞秒激光在生物组织中的消融会产生高荧光化合物，这些化合物对于对消融组织进行内在标记以及实现成像引导的激光显微手术具有重要意义。在本研究中，我们使用拉曼光谱和透射电子显微镜分析了飞秒激光消融组织的分子结构。结果表明，尽管激光消融导致了碳化，但在消融组织中未发现高荧光纳米结构。此外，我们发现新形成化合物的荧光特性在消融部位在空间上是异质的，并且主要荧光信号与在200°C温度下直接加热的组织表现出密切相似性。我们的研究结果表明，新的荧光化合物是通过激光加热效应产生的，其形成机制可能源于美拉德反应，即组织中氨基酸与还原糖之间的化学反应。
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