Köhler Jannis, Ruschke Jegor, Ferenz Katja Bettina, Esen Cemal, Kirsch Michael, Ostendorf Andreas
Applied Laser Technologies, Ruhr-Universität Bochum, Universitätsstraße 150, 44801 Bochum, Germany.
Institut für Physiologische Chemie, Universität Duisburg-Essen, Universitätsklinikum Essen, Hufelandstraße 55, 45147 Essen, Germany.
Biomed Opt Express. 2018 Jan 23;9(2):743-754. doi: 10.1364/BOE.9.000743. eCollection 2018 Feb 1.
Albumin-derived perfluorocarbon-based capsules are promising as artificial oxygen carriers with high solubility. However, these capsules have to be studied further to allow initial human clinical tests. The aim of this paper is to provide and characterize a holographic optical tweezer to enable contactless trapping and moving of individual capsules in an environment that mimics physiological (in vivo) conditions most effectively in order to learn more about the artificial oxygen carrier behavior in blood plasma without recourse to animal experiments. Therefore, the motion behavior of capsules in a ring shaped or vortex beam is analyzed and optimized on account of determination of the optical forces in radial and axial direction. In addition, due to the customization and generation of dynamic phase holograms, the optical tweezer is used for first investigations on the aggregation behavior of the capsules and a statistical evaluation of the bonding in dependency of different capsule sizes is performed. The results show that the optical tweezer is sufficient for studying individual perfluorocarbon-based capsules and provide information about the interaction of these capsules for future use as artificial oxygen carriers.
基于白蛋白的全氟化碳胶囊有望成为具有高溶解度的人工氧载体。然而，这些胶囊必须进一步研究，以便进行初步人体临床试验。本文的目的是提供并表征一种全息光镊，以在最有效地模拟生理（体内）条件的环境中实现对单个胶囊的非接触式捕获和移动，从而在无需进行动物实验的情况下更多地了解人工氧载体在血浆中的行为。因此，考虑到径向和轴向光学力的测定，对胶囊在环形或涡旋光束中的运动行为进行了分析和优化。此外，由于定制和生成动态相位全息图，光镊被用于对胶囊聚集行为的首次研究，并对不同胶囊尺寸依赖性的结合进行了统计评估。结果表明，光镊足以用于研究单个基于全氟化碳的胶囊，并为这些胶囊作为人工氧载体的未来应用提供有关其相互作用的信息。
