Wang Ai-Hua, Ma Li-Man, Fan Shan-Shan, Liu Guang-Xue, Xu Feng, Shang Ming-Ying, Cai Shao-Qing
Department of Natural Medicines, School of Pharmaceutical Sciences, Peking University, Beijing 100191, China.
National Committee on the Assessment of the Protected Traditional Chinese Medicinal Products, Beijing 100070, China.
Zhongguo Zhong Yao Za Zhi. 2018 Jan;43(1):123-133. doi: 10.19540/j.cnki.cjcmm.20171027.003.
This experiment was performed to analyze and identify the chemical constituents of Sinopodophylli Fructus by HPLC-DAD-ESI-IT-TOF-MSn. The analysis was performed on an Agilent Zorbax SB-C₁₈ (4.6 mm×250 mm, 5 μm) column.The mobile phase consisted of 0.1% formic acid was used for gradient at a flow rate of 1.0 mL·min⁻¹. Electrospray ionization ion trap time-of-flight multistage mass spectrometry was applied for qualitative analysis under positive and negative ion modes. The results indicated that 54 compounds consisted of 18 lignans and 36 flavonoids from Xiaoyelian had been detected by their HRMS data, the information of literature and reference substance. Among them, 27 compounds were reported in Sinopodophylli Fructus for the first time. In conclusion, an HPLC-DAD-ESI-IT-TOF-MSn method was established to qualitative analysis of Xiaoyelian in this study, which will provide the evidence for evaluating the quality of Xiaoyelian herbs, clarifying the mechanism, and guiding the development of pharmacological active ingredients.
本实验旨在通过高效液相色谱-二极管阵列检测-电喷雾电离-离子阱-飞行时间多级质谱（HPLC-DAD-ESI-IT-TOF-MSn）对小连翘果实的化学成分进行分析和鉴定。分析在安捷伦Zorbax SB-C₁₈（4.6 mm×250 mm，5 μm）色谱柱上进行。流动相为含0.1%甲酸的溶液，采用梯度洗脱，流速为1.0 mL·min⁻¹。采用电喷雾电离离子阱飞行时间多级质谱在正离子和负离子模式下进行定性分析。结果表明，通过高分辨质谱数据、文献信息和对照品，从小连翘中检测出54种化合物，其中包括18种木脂素和36种黄酮类化合物。其中，有27种化合物首次在小连翘果实中被报道。综上所述，本研究建立了一种HPLC-DAD-ESI-IT-TOF-MSn方法用于小连翘的定性分析，这将为评价小连翘药材质量、阐明作用机制以及指导药理活性成分开发提供依据。
