Ling Yun, Zhang Qing, Zhu Dan-dan, Chen Fei, Kong Xiu-hua, Liao Liang
Department of Pharmaceutical and Life Sciences, Jiujiang University, Jiujiang 332005, People's Republic of China
Department of Pharmaceutical and Life Sciences, Jiujiang University, Jiujiang 332005, People's Republic of China.
J Chromatogr Sci. 2016 Feb;54(2):148-57. doi: 10.1093/chromsci/bmv119. Epub 2015 Aug 26.
Fructus Akebiae (FA), the dry fruit of Akebia quinata (THUNB.) DECNE., possesses potent antidepressant properties. Owing to the structural complexity, high polarity and thermal lability in plants, it is difficult and time-consuming to analyze the major chemical constituents by traditional strategies that involve extraction, isolation, purification and identification by chemical manipulations and spectroscopic methods. In this study, a high-performance liquid chromatography coupled with electrospray ionization-quadrupole-time-of-flight mass spectrometry (HPLC-ESI-Q-TOF-MS-MS) method was established for quickly identifying the chemical constituents in the extract of Fructus Akebiae. The main saponin components in the extract of Fructus Akebiae were detected with the HPLC-ESI-Q-TOF-MS-MS in negative-ion mode. These components were further analyzed by MS(2) spectra, and compared with the corresponding reference substances and literature data. Nineteen saponins in the extract of Fructus Akebiae were well separated in one run. The new method is accurate and rapid. It can be used to identify the main chemical constituents in the extract of Fructus Akebiae and can be suitable for the quality control of Fructus Akebiae.
预知子（FA），即木通科植物木通Akebia quinata (THUNB.) DECNE. 的干燥果实，具有显著的抗抑郁特性。由于植物中化学成分结构复杂、极性高且具有热不稳定性，采用传统策略（包括通过化学操作和光谱方法进行提取、分离、纯化和鉴定）分析其主要化学成分既困难又耗时。本研究建立了一种高效液相色谱-电喷雾电离-四极杆-飞行时间质谱联用（HPLC-ESI-Q-TOF-MS-MS）方法，用于快速鉴定预知子提取物中的化学成分。采用HPLC-ESI-Q-TOF-MS-MS负离子模式检测预知子提取物中的主要皂苷成分。通过MS(2) 谱对这些成分进行进一步分析，并与相应的对照品和文献数据进行比较。预知子提取物中的19种皂苷在一次进样中得到了良好分离。该新方法准确、快速，可用于鉴定预知子提取物中的主要化学成分，适用于预知子的质量控制。
