Jun-Chao D U, Xiang-Lan P U, Kai-Lei Fan, Yan-Juan Zhai, Yu-Jing Zhang, Xie-He Wang, Sheng-Jun Chen, Song L I, Yun-Tian Zhang, Ke-Ji Chen, Jun Peng, Yun-Ming X U
Jiangyin Tianjiang Pharmaceutical Co., Ltd. Jiangyin 214434, China Key Technology Laboratory for Preparation and Quality Control of Traditional Chinese Medicine Granules in Jiangsu Jiangyin 214434, China Jiangsu Traditional Chinese Medicine Formula Granule Engineering Technology Center Jiangyin 214434, China Jiangsu Traditional Chinese Medicine Formula Granule Engineering Research Center Jiangyin 214434, China.
Jiangyin Tianjiang Pharmaceutical Co., Ltd. Jiangyin 214434, China Key Technology Laboratory for Preparation and Quality Control of Traditional Chinese Medicine Granules in Jiangsu Jiangyin 214434, China Jiangsu Traditional Chinese Medicine Formula Granule Engineering Technology Center Jiangyin 214434, China Jiangsu Traditional Chinese Medicine Formula Granule Engineering Research Center Jiangyin 214434, China Academy of Integrative Medicine, Fujian University of Traditional Chinese Medicine Fuzhou 350122, China.
Zhongguo Zhong Yao Za Zhi. 2020 Oct;45(20):4909-4917. doi: 10.19540/j.cnki.cjcmm.20200622.303.
To establish the quantitative analysis multi-components with a single-marker(QAMS) method for six components and fingerprint of standard decoction of Gastrodiae Rhizoma, verify the accuracy and feasibility of the method, and evaluate the quality of standard decoction. Based on UPLC with gastrodin as the internal standard, relative correction factors of p-hydroxybenzyl alcohol, parishin E, parishin B, parishin C, parishin A and gastrodin were determined by investigating the column temperature, flow rate, chromatographic columns and multi-point concentration correction. The total contents in 18 batches of standard decoction of Gastrodiae Rhizoma and the similarity were determined to calculate the similarity. The results of standard curve method, external standard one-point method and quantitative analysis multi-components with a single-marker(QAMS) were compared, and the results showed that there was no significant difference among these three methods. By analyzing the results of standard decoctions from different origins, it can be seen that the quality of Gastrodia standard decoctions derived from Anhui and Yunnan was better, followed by Shaanxi and Hubei, and relatively poor in Gansu, with similarities all above 0.90 in the fingerprints. Therefore, the QAMS method that can measure the contents of gastrodin, p-hydroxybenzyl alcohol, parishin E, parishin B, parishin C and parishin A in standard decoction of Gastrodiae Rhizoma combined with fingerprint is accurate, feasible and fast, which can be used to evaluate the quality of standard decoction of Gastrodiae Rhizoma, and also provide a reference for the research on the quality standards of raw materials for Gastrodiae Rhizoma prepared slices and alike.
建立天麻标准汤剂中6种成分及指纹图谱的一测多评（QAMS）方法，验证该方法的准确性和可行性，并评价标准汤剂的质量。以天麻素为内标，基于超高效液相色谱法，通过考察柱温、流速、色谱柱及多点浓度校正等，测定对羟基苯甲醇、派立辛E、派立辛B、派立辛C、派立辛A与天麻素的相对校正因子。测定18批次天麻标准汤剂的总含量及相似度并计算相似度。比较标准曲线法、外标一点法与一测多评（QAMS）法的结果，结果表明这3种方法之间无显著差异。通过分析不同产地标准汤剂的结果可知，安徽和云南产天麻标准汤剂质量较好，其次为陕西和湖北，甘肃相对较差，指纹图谱相似度均在0.90以上。因此，结合指纹图谱可测定天麻标准汤剂中天麻素、对羟基苯甲醇、派立辛E、派立辛B、派立辛C和派立辛A含量的QAMS方法准确、可行且快速，可用于评价天麻标准汤剂的质量，也为天麻炮制饮片等原料的质量标准研究提供参考。
