[高效液相色谱-二极管阵列检测器双波长法同时测定柴胡中5种皂苷]

[Simultaneous determination of five saponins in Bupleuri Radix by HPLC-DAD dual wavelength method].

作者信息

Liu Yu-Mei, Zhou An, Yu Nian-Jun, Han Rong-Chun, Zhang Wei, Zhu Yue-Jian, Cao Yong, Li Xiang-Yu, Peng Dai-Yin

机构信息

The Experimental Research Center,School of Pharmacy,Anhui University of Chinese Medicine, Hefei 230038， China.

Anhui Puren Prepared Slices of Chinese Crude, Bozhou 236800, China.

出版信息

Zhongguo Zhong Yao Za Zhi. 2018 Jan;43(2):363-368. doi: 10.19540/j.cnki.cjcmm.20171027.001.

DOI:10.19540/j.cnki.cjcmm.20171027.001

PMID:29552856

Abstract

Epoxy ether type and isophthalene type saponin are the main saponins of Bupleurum chinense. However,due to the difference of their UV spectrum,there is no quantitative method for simultaneous determination of these two kinds of saponins. In this paper,a dual-wavelength high performance liquid chromatography(HPLC) was developed for simultaneous determination of five saponins in epoxidized ether(saikosaponin a,c,d) and isosorbide type(saikosaponin b1,b2). The mobile phase was eluted with acetonitrile-water(0.1% phosphoric acid) gradient at a column temperature of 30 °C and a flow rate of 1.0 mL·min⁻¹. The detection wavelengths were 208 nm for saikosaponins a,c, and d, and 254 nm for saikosaponins b₁ and b₂. The results showed that the separation of five kinds of saikosaponin was good, with the linear range of 9.70-1 935.00(=0.999 4),8.20-1 380.00(=0.999 3),6.90-1 640.00(=0.999 0),5.25-630.00(=0.999 4), and 5.15-618.00 mg·L⁻¹(=0.999 5), respectively. The average recoveries were 97.70%-100.2% and the RSD was less than 3%(=6). The method is simple,rapid and reproducible. It can be used for the determination of five kinds of saikosaponins in B. chinense.

摘要

环氧醚型和异苯二甲酸型皂苷是柴胡的主要皂苷。然而，由于它们紫外光谱的差异，尚无同时测定这两种皂苷的定量方法。本文建立了一种双波长高效液相色谱法（HPLC），用于同时测定环氧醚型（柴胡皂苷a、c、d）和异山梨醇型（柴胡皂苷b1、b2）中的5种皂苷。流动相采用乙腈 - 水（0.1%磷酸）梯度洗脱，柱温30℃，流速1.0 mL·min⁻¹。柴胡皂苷a、c、d的检测波长为208 nm，柴胡皂苷b₁和b₂的检测波长为254 nm。结果表明，5种柴胡皂苷分离良好，线性范围分别为9.70 - 1935.00（r = 0.999 4）、8.20 - 1380.00（r = 0.999 3）、6.90 - 1640.00（r = 0.999 0）、5.25 - 630.00（r = 0.999 4）和5.15 - 618.00 mg·L⁻¹（r = 0.999 5）。平均回收率为97.70% - 100.2%，相对标准偏差小于3%（n = 6）。该方法简便、快速、重现性好，可用于柴胡中5种柴胡皂苷的测定。